Aniseed - Cis-regulation

Region 'REG00001304'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:4425113-4425279|Wnttun5

Short name

Wnttun5_-863/-697

Region ID

REG00001304

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Izumi Oda-Ishii (2018-03-15)

Annotator

Marion Gueroult-Bellone (2018-03-15)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This sequence sems to be responsible for restricting Wnttun enhancer activity to the posterior vegetal domain. When it is deleted from the 300pb enhancer, new anterior vegetal activity is observed.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:4425113-4425410|Wnttun5 by deletion

Cirobu.REG.KH2012.C9:4424417-4427405|Wnttun5 REG00001119 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C9:4424417-4425816|Wnttun5 REG00001120 [ regulatory_region (extended_promoter) ]
  - Cirobu.REG.KH2012.C9:4424417-4425395|Wnttun5 REG00001121 [ regulatory_region (extended_promoter) ]
  - Cirobu.REG.KH2012.C9:4425113-4425410|Wnttun5 REG00001299 [ regulatory_region (enhancer) ]
    - Cirobu.REG.KH2012.C9:4425151-4425410|Wnttun5 REG00001300 [ regulatory_region (enhancer) ]
      - Cirobu.REG.KH2012.C9:4425209-4425410|Wnttun5 REG00001301 [ regulatory_region (enhancer) ]
        
        Cirobu.REG.KH2012.C9:4425269-4425410|Wnttun5 REG00001302 [ regulatory_region (enhancer) ]
    - Cirobu.REG.KH2012.C9:4425113-4425279|Wnttun5 REG00001304 [ regulatory_region (enhancer) ]

Regulated gene(s)

KH2012:KH.C9.257 (WNT2; WNT4; WNT6)

Construct(s) made to test this region

CONS00001324: p.Cirobu.REG.KH2012.C8:2621713-2622953_(Cirobu.REG.KH2012.C9:4425113-4425279)x4>GFP

Show in situ data

Overview of functional TF binding sites in this region

4,425,1134,425,279

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [4,425,113 / 4,425,279] on scaffold KhC9

165pb sequence located 700pb upstream of Wnttun5 (KH.C9.257).

Show sequence

Length: 167

taatatacccggacgagtctcggcctcccaataggtggtcggcggcgatcgcaatcgtaa
ttgctcgattccaacccctgcccagcactaggggcgaggaaaccgccaattatcaaatag
atcagtcgcttgcgcatccccgtatgcggagatcggccttgaaataa

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045