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CIS-REGULATION

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  1. Region 'REG00001302'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:4425269-4425410|Wnttun5

Short name

Wnttun5_-994/-853

Region ID

REG00001302

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Izumi Oda-Ishii (2018-03-15)

Annotator

Marion Gueroult-Bellone (2018-03-15)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This deletion affects the activity pattern : in addition to the posterior vegetal domain activity of the 300pb enhancer, anterior vegetal activity is also observed.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:4425209-4425410|Wnttun5 by deletion

Regulated gene(s)

KH2012:KH.C9.257 (WNT2; WNT4; WNT6)

Construct(s) made to test this region

CONS00001322: p.(Cirobu.REG.KH2012.C9:4425269-4425410)x4>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,425,2694,425,410

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a taaac [4,425,340 / 4,425,345] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.
2 TCF/LEF n/a caaa [4,425,387 / 4,425,391] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.

Sequence

Aniseed Coordinates: [4,425,269 / 4,425,410] on scaffold KhC9

140pb sequence located upstream of Wnttun5 (KH.C9.257). It contains two Tcf7 putative binding sites.

Show sequence

Length: 142


ccttgaaataacaaactgccgacaaaactcggcgggccaattaaagcccctgtagctcgc
aaatgcagagataaacaaacaagctgctcgcgtgtggacctgtaattacggcagcgatca
aacgacgacgatcttgctgcag

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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