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CIS-REGULATION

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  1. Region 'REG00001299'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:4425113-4425410|Wnttun5

Short name

Wnttun5_-994/-694

Region ID

REG00001299

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Izumi Oda-Ishii (2018-03-15)

Annotator

Marion Gueroult-Bellone (2018-03-15)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This deletion does not affect the activity pattern in the posterior vegetal domain.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:4424417-4425816|Wnttun5 by deletion

Regulated gene(s)

KH2012:KH.C9.257 (WNT2; WNT4; WNT6)

Construct(s) made to test this region

CONS00001319: p.(Cirobu.REG.KH2012.C9:4425113-4425410)x4>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,425,1134,425,410

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a taaac [4,425,340 / 4,425,345] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.
2 TCF/LEF n/a caaa [4,425,387 / 4,425,391] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.

Sequence

Aniseed Coordinates: [4,425,113 / 4,425,410] on scaffold KhC9

300pb sequence located upstream of Wnttun5 (KH.C9.257). It contains two Tcf7 putative binding sites.

Show sequence

Length: 298


taatatacccggacgagtctcggcctcccaataggtggtcggcggcgatcgcaatcgtaa
ttgctcgattccaacccctgcccagcactaggggcgaggaaaccgccaattatcaaatag
atcagtcgcttgcgcatccccgtatgcggagatcggccttgaaataacaaactgccgaca
aaactcggcgggccaattaaagcccctgtagctcgcaaatgcagagataaacaaacaagc
tgctcgcgtgtggacctgtaattacggcagcgatcaaacgacgacgatcttgctgcag

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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