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CIS-REGULATION

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  1. Region 'REG00001295'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S654:26922-27123_μC|Tbx6.b

Short name

Tbx6.b_-189_μC

Region ID

REG00001295

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Izumi Oda-Ishii (2018-03-13)

Annotator

Marion Gueroult-Bellone (2018-03-13)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This substitution does not affect level of expression but the WT expression pattern (posterior vegetal domain) is affeceted as this synthetic enhnacer also drives activity in the anterior vegetal domain.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.S654:26922-27123|Tbx6.b by substitution

Regulated gene(s)

KH2012:KH.S654.3 (TBX2; TBX4; TBX6)

Construct(s) made to test this region

CONS00001315: p.(Cirobu.REG.KH2012.S654:26922-27123_μC)4x>GFP

Show in situ data
Overview of functional TF binding sites in this region

26,92227,124

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a ctttg [26,980 / 26,985] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.
2 TCF/LEF n/a aaaac [27,075 / 27,080] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.

Sequence

Aniseed Coordinates: [26,922 / 27,124] on scaffold KhS654

Mutation of 15pb of the 200pb enhancer of Tbx6.b (KH.S645.3). Mutated sequence is shown in capital letters.

Show sequence

Length: 203


cggaatgaggtactgtgaaactaaacccattttatagcgagactcgttttcgatgtcact
ttgatcttaacgagtcattaacaaggacaactgcttcctgataagactccgaactctTTC
ATCAGCCTACACctagcgcatgcagaacactccaaaactcagtgtacgcataccgtcttg
catgtggcgcgttggtttgataa

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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