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CIS-REGULATION

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  1. Region 'REG00001129' > 
  2. Region 'REG00001259'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C7:40900-40993_Fox2mut|Noe2; Olm2A/B

Short name

Noe/Olm_CRM112_94pb_Fox2mut

Region ID

REG00001259

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Diana S. José-Edwards (2017-09-10)

Annotator

Marion Gueroult-Bellone (2017-09-10)

Curator

Marion Gueroult-Bellone (2018-05-09)

View position in WashU

Description

Notochord activity of Noe2/Olm2A/B 94pb enhancer is strongly decreased by the mutation of the second Fox putative binding sites. This mutation also induces ectopic weak activity in tail muscle and central nervous system.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C7:40900-40993|Noe2; Olm2A/B by substitution

Regulated gene(s)

KH2012:KH.C7.500 (OLFM2; OLFML2A; OLFML2B)

Construct(s) made to test this region

CONS00001262: p.Cirobu.REG.KH2012.C7:40900-40993_Fox2mut>LacZ

Show in situ data
Overview of functional TF binding sites in this region

40,90040,993

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a acaaata [40,934 / 40,941] First Fox putative binding site of Noe2/Olm2A/B 94pb enhancer. This putative binding site is deemed dispensable through truncations experiments and do not evidently contribute to the notochord activity of the enhancer.
2 Homeobox n/a atta [40,966 / 40,970] Homeodomain putative binding site of Noe2/Olm2A/B 94pb enhancer. It is required for notochord expression.
3 bZIP n/a tgac [40,987 / 40,991] AP1 putative binding site of Noe2/Olm2A/B 94pb enhancer. It is required for notochord expression.

Sequence

Aniseed Coordinates: [40,900 / 40,993] on scaffold KhC7

The second Fox binding site is mutated. Fox2 : ACAAACA replaced by AACCCAA

Show sequence

Length: 94


gaatgttacgcgttagaaagtcgaaacagggcgaacaaatactcttcggttcggcactga
tgcgaaattagaacccaagtctcgctctgaccgc

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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