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CIS-REGULATION

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  1. Transcript 'Mooccu.CG.ELv1_2.S38...' > 
  2. Region 'REG00001255'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C7:40900-40980|Noe2; Olm2A/B

Short name

Noe/Olm_CRM112_81pb

Region ID

REG00001255

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Diana S. José-Edwards (2017-09-10)

Annotator

Marion Gueroult-Bellone (2017-09-10)

Curator

Marion Gueroult-Bellone (2018-05-09)

View position in WashU

Description

This 81pb region, located upstream of Noe2; Olm2A/B (KH.C7.500), drives weak reporter gene expression in notochord and tail muscle, and strong expression in mesenchyme. It contains two Fox, and a HD putative binding sites, and lacks an AP1 site compared to the 94pb minimal enhancer.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C7:40900-40993|Noe2; Olm2A/B by deletion

Regulated gene(s)

KH2012:KH.C7.500 (OLFM2; OLFML2A; OLFML2B)

Construct(s) made to test this region

CONS00001258: p.Cirobu.REG.KH2012.C7:40900-40980>LacZ

Show in situ data
Overview of functional TF binding sites in this region

40,90040,980

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Fox n/a acaaata [40,934 / 40,941] First Fox putative binding site of Noe2/Olm2A/B 94pb enhancer. This putative binding site is deemed dispensable through truncations experiments and do not evidently contribute to the notochord activity of the enhancer.
2 Homeobox n/a atta [40,966 / 40,970] Homeodomain putative binding site of Noe2/Olm2A/B 94pb enhancer. It is required for notochord expression.
3 Fox n/a acaaaca [40,971 / 40,978] Second Fox putative binding site of Noe2/Olm2A/B 94pb enhancer. It is required for notochord expression.

Sequence

Aniseed Coordinates: [40,900 / 40,980] on scaffold KhC7

This sequence was retrieved using ANISEED Blast tool, thanks to the information provided in FigS2.d and TableS5 of Jose-Edwards et al. 2015.

Show sequence

Length: 81


gaatgttacgcgttagaaagtcgaaacagggcgaacaaatactcttcggttcggcactga
tgcgaaattagacaaacagtc

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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