- Region 'REG00001235'
Cis-regulatory Region
Name
Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa-OCb)|Onecut_HNF6
Short name
Onecut/HNF6 (-935/-674)µ_Ngn-OCa-OCb
Region ID
REG00001235
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(enhancer)
Author
Maria Rosa Pezzotti (2017-08-06)
Annotator
Marion Gueroult-Bellone (2017-08-06)
Curator
Delphine Dauga (2017-09-06)
This sequence derives from the 260pb cis-regulatory sequence of Onecut/HNF6 (REG00001223) which contains 2 Onecut binding sites (-746/-730pb and -705/-689pb from TSS) and one Neurogenin binding site (-847/-835pb from TSS). Mutation of these three binding sites causes the loss of specific enhancer activity.
Modification of Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 by substitution
- Cirobu.REG.KH2012.C6:403105-405698|Onecut_HNF6 REG00001211 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:404848-405698|Onecut_HNF6 REG00001212 [ inactive_region ]
- Cirobu.REG.KH2012.C6:404401-404922|Onecut_HNF6 REG00001213 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403105-404485|Onecut_HNF6 REG00001214 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403974-404485|Onecut_HNF6 REG00001215 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403548-403985|Onecut_HNF6 REG00001216 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403105-403572|Onecut_HNF6 REG00001217 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403733-404043|Onecut_HNF6 REG00001218 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 REG00001223 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403839-404043|Onecut_HNF6 REG00001224 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-403940|Onecut_HNF6 REG00001228 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn)|Onecut_HNF6 REG00001229 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCa)|Onecut_HNF6 REG00001230 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCb)|Onecut_HNF6 REG00001231 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa)|Onecut_HNF6 REG00001232 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCb)|Onecut_HNF6 REG00001233 [ inactive_region ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCa-OCb)|Onecut_HNF6 REG00001234 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa-OCb)|Onecut_HNF6 REG00001235 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403733-403838|Onecut_HNF6 REG00001225 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 REG00001223 [ regulatory_region (extended_promoter) ]
CONS00001237: p.Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa-OCb)>LacZ
403,782404,043
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
|---|
Aniseed Coordinates: [403,782 / 404,043] on scaffold KhC6
This fragment extending from the position 935 to 674 upstream of the translation start site was prepared by site-directed mutagenesis from the 260pb Onecut/HNF6 enhancer (REG00001223). The Neurogenin binding site (-847/-835pb from TSS) and the two Onecut binding sites (-746/-730pb and -705/-689pb from TSS) were mutated (respectively replaced by gggcggtggggtg, aattgtggggggttgga and aattgtggggggttgga).
Show sequence
Length: 262
ctgactaaactgttttataggggggtatttttctttccaaggcttgaacttgtcttaatt
gtggggggttggaatttaaacaaattttcgatcagtcctttacggttgagctccaagctc
gaatggaagactggtgccgtactcgtatgggagtaagcaatgggcggtggggtgcgtttt
tctgttcggcttatatacgagtggttcgcgtcggcgcgcggtttgagcaaaaacaatagg
accgctgtcgaaaacaaggcga
