- Region 'REG00001233'
Cis-regulatory Region
Name
Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCb)|Onecut_HNF6
Short name
Onecut/HNF6 (-935/-674)µ_Ngn-OCb
Region ID
REG00001233
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
inactive_region
Author
Maria Rosa Pezzotti (2017-08-06)
Annotator
Marion Gueroult-Bellone (2017-08-06)
Curator
Delphine Dauga (2017-09-06)
This sequence derives from the 260pb cis-regulatory sequence of Onecut/HNF6 (REG00001223) which contains 2 Onecut binding sites (-746/-730pb and -705/-689pb from TSS) and one Neurogenin binding site (-847/-835pb from TSS). Enhancer activity is lost by mutating the neurogenin and second Onecut binding site.
Modification of Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 by substitution
- Cirobu.REG.KH2012.C6:403105-405698|Onecut_HNF6 REG00001211 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:404848-405698|Onecut_HNF6 REG00001212 [ inactive_region ]
- Cirobu.REG.KH2012.C6:404401-404922|Onecut_HNF6 REG00001213 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403105-404485|Onecut_HNF6 REG00001214 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403974-404485|Onecut_HNF6 REG00001215 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403548-403985|Onecut_HNF6 REG00001216 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403105-403572|Onecut_HNF6 REG00001217 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403733-404043|Onecut_HNF6 REG00001218 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 REG00001223 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403839-404043|Onecut_HNF6 REG00001224 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-403940|Onecut_HNF6 REG00001228 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn)|Onecut_HNF6 REG00001229 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCa)|Onecut_HNF6 REG00001230 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCb)|Onecut_HNF6 REG00001231 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa)|Onecut_HNF6 REG00001232 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCb)|Onecut_HNF6 REG00001233 [ inactive_region ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCa-OCb)|Onecut_HNF6 REG00001234 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa-OCb)|Onecut_HNF6 REG00001235 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403733-403838|Onecut_HNF6 REG00001225 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 REG00001223 [ regulatory_region (extended_promoter) ]
CONS00001235: p.Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCb)>LacZ
403,782404,043
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | Homeobox | n/a | aattgtagattgtttga | [403,838 / 403,855] | This Onecut/HNF6 binding site is one of the three identified binding sites of HNF6/Onecut minimal enhancer (the others are a Neurogenin and another HNF6/Onecut sites). It is located at -746/-730pb from HNF6/Onecut TSS. The mutational analysis not only indicates that all the three identified binding sites (Ngn, OCa and OCb) are necessary for Onecut activation in its endogenous territories but also that a cooperation between the two OC sites for the maintenance of its expression exists. |
Aniseed Coordinates: [403,782 / 404,043] on scaffold KhC6
This fragment extending from the position 935 to 674 upstream of the translation start site was prepared by site-directed mutagenesis from the 260pb Onecut/HNF6 enhancer (REG00001223). The Neurogenin binding site (-847/-835pb from TSS) and the second Onecut binding site (-705/-689pb from TSS) were mutated (respectively replaced by gggcggtggggtg and tataggggggtattttt).
Show sequence
Length: 262
ctgactaaactgttttataggggggtatttttctttccaaggcttgaacttgtcttaatt
gtagattgtttgaatttaaacaaattttcgatcagtcctttacggttgagctccaagctc
gaatggaagactggtgccgtactcgtatgggagtaagcaatgggcggtggggtgcgtttt
tctgttcggcttatatacgagtggttcgcgtcggcgcgcggtttgagcaaaaacaatagg
accgctgtcgaaaacaaggcga
