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CIS-REGULATION

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  1. Region 'REG00001220'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C6:403340-403572|Onecut_HNF6

Short name

Onecut/HNF6 (-464/-232)

Region ID

REG00001220

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Maria Rosa Pezzotti (2017-08-06)

Annotator

Marion Gueroult-Bellone (2017-08-06)

Curator

Delphine Dauga (2017-09-06)

View position in WashU

Description

This sequence derives from the 460pb cis-regulatory sequence of Onecut/HNF6 (REG00001217) active in the ectopic mesenchyme in Ciona intestinalis tailbuds and larvae. The activity of the daughter sequence is similar to the larger one.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C6:403105-403572|Onecut_HNF6 by deletion

Regulated gene(s)

KH2012:KH.C6.185 (ONECUT1; ONECUT2; ONECUT3)

Construct(s) made to test this region

CONS00001222: p.Cirobu.REG.KH2012.C6:403340-403572>LacZ

Show in situ data
Overview of functional TF binding sites in this region

403,340403,572

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [403,340 / 403,572] on scaffold KhC6

This fragment extending from the position 464 to 232 upstream of the translation start site was PCR-amplified from the 2.6kb Onecut/HNF6 enhancer (REG00001211).

Show sequence

Length: 233

aatgatgcttcagatactctgctcatcattagaacttgaaattaaaactaacttgttttt
caacatcgtcaaaacttagacgcataaaatgtacgcgcatataataagtcaaaaatgtta
caaagttgccaaatatttaaaaattggaatattatgtttaacttaaatgataccattttt
tgatttatgatatatggtttagaaattgacaacgaactccgaagaattcaacg

Reference(s)

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

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