- Region 'REG00001214'
Cis-regulatory Region
Name
Cirobu.REG.KH2012.C6:403105-404485|Onecut_HNF6
Short name
Onecut/HNF6 (-1377/-4)
Region ID
REG00001214
Status
Curated
Origin
natural region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(extended_promoter)
Author
Maria Rosa Pezzotti (2017-08-06)
Annotator
Marion Gueroult-Bellone (2017-08-06)
Curator
Delphine Dauga (2017-09-06)
Modification of Cirobu.REG.KH2012.C6:403105-405698|Onecut_HNF6 by deletion
- Cirobu.REG.KH2012.C6:403105-405698|Onecut_HNF6 REG00001211 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:404848-405698|Onecut_HNF6 REG00001212 [ inactive_region ]
- Cirobu.REG.KH2012.C6:404401-404922|Onecut_HNF6 REG00001213 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403105-404485|Onecut_HNF6 REG00001214 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403974-404485|Onecut_HNF6 REG00001215 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403548-403985|Onecut_HNF6 REG00001216 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403105-403572|Onecut_HNF6 REG00001217 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403733-404043|Onecut_HNF6 REG00001218 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 REG00001223 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C6:403839-404043|Onecut_HNF6 REG00001224 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-403940|Onecut_HNF6 REG00001228 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn)|Onecut_HNF6 REG00001229 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCa)|Onecut_HNF6 REG00001230 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCb)|Onecut_HNF6 REG00001231 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa)|Onecut_HNF6 REG00001232 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCb)|Onecut_HNF6 REG00001233 [ inactive_region ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_OCa-OCb)|Onecut_HNF6 REG00001234 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043(µ_Ngn-OCa-OCb)|Onecut_HNF6 REG00001235 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403733-403838|Onecut_HNF6 REG00001225 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.C6:403782-404043|Onecut_HNF6 REG00001223 [ regulatory_region (extended_promoter) ]
403,105404,485
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | Homeobox | n/a | tataattgaatattttt | [403,797 / 403,814] | This Onecut/HNF6 binding site is one of the three identified binding sites of HNF6/Onecut minimal enhancer (the others are a Neurogenin and another HNF6/Onecut sites). It is located at -705/-689pb from HNF6/Onecut TSS. The mutational analysis not only indicates that all the three identified binding sites (Ngn, OCa and OCb) are necessary for Onecut activation in its endogenous territories but also that a cooperation between the two OC sites for the maintenance of its expression exists. |
| 2 | Homeobox | n/a | aattgtagattgtttga | [403,838 / 403,855] | This Onecut/HNF6 binding site is one of the three identified binding sites of HNF6/Onecut minimal enhancer (the others are a Neurogenin and another HNF6/Onecut sites). It is located at -746/-730pb from HNF6/Onecut TSS. The mutational analysis not only indicates that all the three identified binding sites (Ngn, OCa and OCb) are necessary for Onecut activation in its endogenous territories but also that a cooperation between the two OC sites for the maintenance of its expression exists. |
| 3 | bHLH | n/a | gggccatctgctg | [403,943 / 403,956] | This Neurogenin binding site is one of the three identified binding sites of HNF6/Onecut minimal enhancer (the others are two HNF6/Onecut sites). It is located at -847/-835pb from HNF6/Onecut TSS. At neurula stage, Neurogenin is necessary for enhancer activation in the Onecut territories. Then, neurogenin seems to be the activator only in the visceral ganglion up to larva stage. |
Aniseed Coordinates: [403,105 / 404,485] on scaffold KhC6
This fragment extending from the position 1377 to 4 upstream of the translation start site was PCR-amplified from the 2.6kb Onecut/HNF6 enhancer (REG00001211).
Show sequence
Length: 1,381
gcatagacgttattctaaaccagcatttaaggatttctcagcgggaatctttgtaaaatt
ttgacgaaaatttcaaattttttaaatctttttgtgaaataactgtaaagcagaaaataa
aagttcaattttattaaagctaaagtcattttaaccaaatcagtattcgttccacagttc
catatatacattaacctaactaggtacttaattgtgatttctcaatgagttattcaatga
tgcttcagatactctgctcatcattagaacttgaaattaaaactaacttgtttttcaaca
tcgtcaaaacttagacgcataaaatgtacgcgcatataataagtcaaaaatgttacaaag
ttgccaaatatttaaaaattggaatattatgtttaacttaaatgataccattttttgatt
tatgatatatggtttagaaattgacaacgaactccgaagaattcaacgtcgccatttatt
tacgatcatgcatgtcgctgcgtatacttatatgctggttatgcacgtaccttttataaa
gtatatataaaattacttttatttagttatatagtcgaaaaaatattatcaaaaatattc
tttttttcgaaaatattttaatttctctcttgcgctatgcgaacttgaatcttagaaact
gtaattaggcctccaaactgactaaactgttttataattgaatatttttctttccaaggc
ttgaacttgtcttaattgtagattgtttgaatttaaacaaattttcgatcagtcctttac
ggttgagctccaagctcgaatggaagactggtgccgtactcgtatgggagtaagcaatgg
gccatctgctgcgtttttctgttcggcttatatacgagtggttcgcgtcggcgcgcggtt
tgagcaaaaacaataggaccgctgtcgaaaacaaggcgatttcgcggtgctttaatataa
cgacattatgccctttttagagttgtattttaaagctactacagcgagtttttgtcgcat
tgtggtaaacgtaacctatgtatgaaaacatttatcgcaaaatataataaagcaaaagta
actttactattacacaaattaatgatacagacttaccaatcgtaggactttagattcatt
ttcatctttttaaaattctgtaaaaaagggaaaaaataaaacactttgatttttttaaat
gatacaataaaagtacatggcgaaagcggtcccatttccgtgggtaaaggtcctattaat
cgtttcaacatgtgcccgaataaatgaacatatggcgtggcatacggacacgacgctcgg
cacagacccggctaatatacaatagacaagactatttcctttattgccctgctacatctc
g
