- Region 'REG00000696' >
- Region 'REG00000002' >
- Region 'REG00000878' >
- Region 'REG00000888' >
- Region 'REG00001203'
Cis-regulatory Region
Name
Cirobu.REG.KH2012.L152:106567-108602::106013-106494|islet
Short name
islet cis-regulatory DNA (Wagner2014)
Region ID
REG00001203
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(complex_region)
CONS00001187: p.Cirobu.REG.KH2012.L152:106567-108602::106013-106494>mCherry
CONS00001191: p.Cirobu.REG.KH2012.L152:106567-108602::106013-106494>YFP-caax
106,013108,546
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
|---|
Aniseed Coordinates: [106,013 / 108,546] on scaffold KhL152
Islet cis- regulatory DNA was pieced together from a 500 bp promoter region and the partial sequence of the first intron cloned upstream. The Islet promoter was amplified with Isl pro XhoF: TAATCTCGAGTTAACTTAACATGGGCG- TGTG and Isl pro NR: TAATGCGGCCGCTTCGTTGATAAAACTT- GTGAAC. The Islet intronic enhancer was amplified with Islet int1a AscF: TAATGGCGCGCCGCCTCGCTTAATTGCGGTAAG and Islet int1a XhR: TAATCTCGAGGCCAAACAAAAACTTTATTTTATTTC
Show sequence
Length: 2,534
GGCGCGCCGCCTCGCTTAATTGCGGTAAGTTTGTGGGTTGTTTAATAAAGTAGGGGGGTT
TGGGTTCGAAATCAGGCCAGGGTTGAAAATCCAGGGATCCCGGGTTCTAATCCAGGTCAG
GGTTGGAGAACCAGGGATCCTGGGTTCGAATTCAGGCCAAGAACTAGCATTGTACGCGTA
AGTGTCTTGAACAAAATTGCGGGTAAGTGCTTAAGCAGGAAAGGCAACAATTAACCTTAG
GATTCAGTATATAGTGCGAAGACGGATACATTATTAATGGGGGGGCGACCTTTGTAAGCG
CCTAACATTGGACTGGGCCGGGGGGTTGGGGCGCGATGTTCGCGATGAAATTGGATTTTT
ATCTTTTGGCAACAAAATTACCGTTCGCGTAGATCGCCATCTTTAAACCGCGCGGCGAAT
ATCTCAAGCCATTGTCACGTCACAATCGGCATTTATTATTTATTTACGTCACAACGGCTT
AATCTGAAGCTGCAAAAGGTATTTGTGACATCACAAAACATAACAATGAAGTTGTGTTAA
AGCATTGTGAATCACAAATACGAGATAATATACATCGCTTATGTCGTCATAACCGCGTCG
ATGCAGCCGCGTAAATCGCACTCAATGTCCCATTCAAGCTGCTACGTCACAATCGCTATT
CTTTCCCCACAATAGACAATGTTAGTTAACAATTTCCCGTGTGTGACGCAACAATTAAAA
CAATAAAGAACAATGCAGTGTATTCTAATACATTGTAGAATGCTCGCGTCATCTTACAGC
GCACACCGCGGTTGCATCGAATGAATAATGCAACAACATATCGACCCGCATTCGATAAAA
TATCAGACTTTTATATTTTCCTTAAAGACCTCAAGTATTTTAACTTTTTTTCGAAAATCT
TTTTTATTTTAATAATATTTTCGCAGTGACGTCATTAATTTATGACATCACCAGTGATAT
CATGACGTCACTCGATCAATATTAGCCAAGAGCAAAAATAGTGGATATTTTTCATTAATT
CTTTATTTGTTTATTTAACCGGAAACAAAATGGCCGCCGGTGACCTGGTTGATTGTGACG
TATATAACACGCAACACACGACTATTATGACGAAACACAGACTTGAATTGAAAATCTAGA
ACCCCGGTCACGTTATGGTAATGCTTATGACGAAATAATTGAAAGTTTTTGCGATTGTGA
CGTCATAATACCTTTATTGTTAGGCGATTGGTTAATTAGGACGCGTGTTCGTTTTTAATT
GTGACGTAATAAACATGAAATTAAACGAGATTGCCAAAGTTAAATGATGCATTATGACAT
CATAATATATAACTTTTATGTGATTCTAGAAAAGACGAAACCGCGAAAGTAGAACAAGAC
GATTGTTAACAAAGCTACGTCATAATCCATAATCAACACGCGAATCGCGTCGCTAAGTAT
CCGCACCTGTGACGTAACAATACTAAGACACAGGCAAACTTCGATATCGCTAAAATATAT
GATTTCTATGCGATCGCGATTAAGTGGTTTTAATTGTTCGCTAGCAAAGTGGTTTGTGGA
GATTAATTTGAGGTAATGTAGTTTAGAACCAACTTATTGTAAATTATCTAAAGTGGTCAC
ATGTATACGCACGTGTATATGGATTAATTAAGGGGGGTCATTAAGTGTAAAGTCGCTAAT
CCCCCTCACCCGTGAGACTTTAGTCATTAACAAAGTGTTTGTTAATTGATCGACAAAGTG
GGTCGCGGTGTAATAGTTGCTGCATTATGACATAACATGCATTATAAATGTGACGTCACA
TTGAATAAACAATCGTCTATATTTGGATTTTAGGACTTGAGATAAAAAAGTTAAAATATG
TTTGGGTCTTGATGGCTTGTAACTTTTGTTGGGGATTATTTTGTTAATTGTTGGGAGGGT
CAGGTTTTTAAGAGCTTTGTTAAGCGTTAAATAAAGAAAGAAAGAAATTTAAATAAATTA
ATTTAATTTTAGATTGAACTTTCTTAAACACAGAAATAAAATAAAGTTTTTGTTTGGCCT
CGAGGGTTAACTTAACATGGGCGTGTGAAATGTAATTAGTTAATGTAGTTAGTCGTATCT
GGATCGGAAGGCATTCATTTGCACTGTAGGCATTGAACTAGGAGGTTCACCACATCGAGG
AATATTTGGTCGCGCTGCGGGGATTGAGGGCCTTGTAGTTGGGGGGAGGTTTGAAAGTTT
CCCGAAGTAATATTAACTTTGTTGTATAGGACCCGTTGTTGCTGTTAGAGAGAATCGGAG
AAAAAGTTTCGAGAGAAGCTTTAGATAAATTGATTCTGTCGCATTCGAGTATTACAACTT
GTGTCTGTGACGTGAGCGAGTGAGGACAATTTACTGTGACGTCATAATAGCCAGCAATAT
AGGGGTGGAAGGTGAATACCCCGCTCTACAAGCGGAGAATTTCGAAATTTCAAACCTGGA
TCCCGGATACTCGCCGCCGTTGGCGGACGACTCAAGGTCGGGGTCGTTCACAAGTTTTAT
CAACGAAGCGCCGC
