Login
Help

CIS-REGULATION

Submit your Data

  1. Molecular Tool 'FoxC>DN_FGFR' > 
  2. Region 'REG00001202'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S605:16789-17833|βγ-crystallin

Short name

βγ-crystallin 1kb upstream

Region ID

REG00001202

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Sebastian M. Shimeld (2017-05-06)

Annotator

Marion Gueroult-Bellone (2017-05-06)

Curator

Delphine Dauga (2017-09-01)

View position in WashU

Description

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.S605:16609-17833|βγ-crystallin by deletion

Regulated gene(s)

KH2012:KH.S605.3 (CRYBB2; CRYGN; CRYGS)

Construct(s) made to test this region

CONS00001186: p.Cirobu.REG.KH2012.S605:16789-17833>GFP

Show in situ data
Overview of functional TF binding sites in this region

16,78917,833

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [16,789 / 17,833] on scaffold KhS605

1kb region upstream beta,gamma-crystallin (KH.S605.3). It mainly drives expression in the palps and occasionnally in the ocellus or the otolith, in cells located adjacent to the ocellus. Ci-beta,gamma-crystallin promoter region cloned in reverse orientation in front of GFP showed no fluorescence.

Show sequence

Length: 1,045

taattcttactgttcggttgaaactcttgaatccactatgacgtcatcatcgtcgctaca
accgctataaacagcgagaaacaaaataaaaacaatcgacttcattacggaagagttggg
tcgaaagtggttgttgcatgaaatatatgtgtgttatatctcggtgctaagtggtacaac
aaacaaataaaacgtctggtttatgagatgctcgcgtggtacgggctatgtgatgtcata
atacctttgtctattatccttgttacgacataattaccatgaaacagcattgtgacgtca
cagtgctgtttgtgttggacaaacaacacgtggtttctacgagggggaatcccctctctg
tcattattacgtatgatgacgtataatatcgattattattcaccatccaaggttatatac
gatatctatttggttttatccgcttggtggcgacatcaatcctcaactacaattggtatt
atgacgtagttcgtagttacgtcacaagtcacacaacaaagaatttattatgagcaaata
aacgaactaatatgacgcaacaataactaacaatttatcacgtgatcgttttatttattt
tgtttgttatgtcataaccacttggtgcttaaaagggagcttatgacgtattaatgaact
ttaattattaaaacaatcttgcttatcgtgctgtgttacgtcataatacatgcattagca
attactttgttttgtcataatatgaattatgtaattatcgtgacgttgtttgttttaaaa
acccatttattattacgtcataatactttagatggttgtgtatgttacgtcataatgtta
attgttgaataaaaatgaagaaacaaagccatgaagaaggcggtttgttataaaacattg
ttgaagggatggaattaattatgttgttaacgtttgaattattgatgtaacaaggacgac
aagtgagcgcaaatcagatttcgtttactttccttctaaccctcctaaccactgcttatt
tcgcattttgtacaatcgaagtttc

Reference(s)

Wagner et al. (2014). Islet is a key determinant of ascidian palp morphogenesis. Development 141(15):3084-92

Shimeld et al. (2005). Urochordate betagamma-crystallin and the evolutionary origin of the vertebrate eye lens. Curr. Biol. 15(18):1684-9

Back to Top