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CIS-REGULATION

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  1. Region 'REG00000370' > 
  2. Transcript 'Moocci.CG.ELv1_2.S45...' > 
  3. Region 'REG00000053' > 
  4. Transcript 'Moocci.CG.ELv1_2.S14...' > 
  5. Region 'REG00001197'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA_AP2-1,2mutation

Short name

Ci-cdc25(-2109/+3) µGATA_AP2-1,2mutation

Region ID

REG00001197

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Yosuke Ogura ()

Annotator

Marion Gueroult-Bellone (2017-05-01)

Curator

Delphine Dauga (2017-05-30)

View position in WashU

Description

This sequence contains the cis-regulatory module CRM2 and CRM1 (as defined by Ogura and Sasakura, 2016). In this variant, both GATA binding sites of CRM1 were mutated (replaced by AATA), and the first AP-2 binding site of CRM2 (ie. the closest to the +1 of transcription) was also mutated (GC turned to GT).

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA by substitution

Regulated gene(s)

KH2012:KH.C5.12 (CDC25A; CDC25B; CDC25C)

Construct(s) made to test this region

CONS00001175: pSP.Cirobu.REG.KH2012.C5:4201966-4204077(µGATA_AP2-1,2 mutated):prCirobu.bpForkhead>Kaede

Show in situ data
Overview of functional TF binding sites in this region

4,201,9664,204,077

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 SOX n/a ttcaacg [4,203,985 / 4,203,992] This binding site is one of the 3 Sox sites of the cis-regulatory module 2 of Cdc25 (KH.C5.12). When all of them are deleted, this CRM is no longer able to drive expression in the epidermis.
2 SOX n/a cattgtt [4,204,030 / 4,204,037] This binding site is one of the 3 Sox sites of the cis-regulatory module 2 of Cdc25 (KH.C5.12). When all of them are deleted, this CRM is no longer able to drive expression in the epidermis.
3 SOX n/a cattgtt [4,204,062 / 4,204,069] This binding site is one of the 3 Sox sites of the cis-regulatory module 2 of Cdc25 (KH.C5.12). When all of them are deleted, this CRM is no longer able to drive expression in the epidermis.

Sequence

Aniseed Coordinates: [4,201,966 / 4,204,077] on scaffold KhC5

In this sequences, two GATA binding sites were mutated and both AP-2 site were mutated (GC turned to GT).

Show sequence

Length: 2,112


catattaaagacaggtgctgaagattgaccacaagtgtataacacccgctagcttacaac
agagtaagctctcaagataccgtatgtctaaaacgcaattagctacttcggatgtcaaat
aacctgaaattctgcaaaagaaaacagtttgcttactcgatcggcgaagatactctgcta
atccacgagatatcggcttatgacaaacgcccataagttacatcgccgatatgacccaat
tccatcggcaatacgaccactatgttattgcggtctttactaagtcataatccaacttta
taacgcttgcgtcgcagaacaactgttgcaataagctccaatggccacaccatatgttac
gctcgaggtataggaggccttttgagtatatacccgacattctaatactactcactcaat
ataatacacaatacaacgtatgtatgtatgctgcatggtactctcttaatcaacacaata
aaatgacatatccaaaaccccaataatactatagccagctaactagtatacgactcttag
attctagttattgtgcaatgaaccgtctagtatcgtcagttaaacaaccatgtaatagca
tagtctctttctctcagtgctcgaggagcattccactttagttttaacgcactacaaact
actatacatacgcaatttctttcagttaccaatacgctatagtcgctatataacgtggta
gcctcacgctacagttatgtgctttagatgtgaatacatccgcctgtcgatactggccac
ttgtcctatatacattggatacggcttatcggcacaaagtaaacaataacaatgcgtagt
atgtggtctgcaatgcgctgctctgggtatactggaacggtactacattgttgtacagta
atgataaagttttacgtagaccagtaactacctagaggtagctgaataggtgaatactgt
ttgactcttatgcttctgtctagtaatatataagagccgaagtctgtctcgctgcttaat
acgcgctcaaactgaaatcccaggcaagatgtctacgattaggaagttgagaaaaaaggc
ccaagagctatgagctaattcccttgcattgtccggtttggacgctaattgatattctga
aggcagctcatgtaaactggtttacgaagcatcttacaatccggccaagctgggacacag
cgtgcgagtgactacctttacctgtgacgcgattaccaagcgagagattaacctttttcc
tttggcggtgagtcgaaaaggaaacataccgctgggctctcgctcggtagcgagtttgct
gctggcaagtaaatcctacgaccaacaatactagcgcaccttggcccggctggatgcagt
caggatgtctcggttggctgtttgtacgctatgcgggcactatggatgcacgcaagtgga
ttaacgggtgggattcttgcttaatatatccgaagttgggaactagttacggattaggca
gcgagtcggttctaatttttatagaaaagtttgctccgcggagatatttacgagaagcac
cacatatatattgacgacgtatttttgcttactactactgtcctactaacgttgcacgaa
aaacacacaactagtcttcctttaactatgcaggcagaacatgagtgaatactagattag
ttttaattaacgaatcggtcactatcctgcactgttgcatattctccaattacatggaat
attgctaataatgcatgtataccgaatcgatctactaacaaattgaactcagctggttgt
gcgctgcatgtgcagttgaatgaatggtgccctgcatcgaaccgcagctgctattttaag
agtgctggtgcagtcgcctttttctcgctttactacaggttcagtgtgttttggtcgtcc
actgataagtagcgaaggccgaagcgtcgcggttcgggccccgaggcacgcactattcta
gcgtcataaataagagctggattcgttctaataggaccgttcaacgaattgcctctcctt
tcacctgtccacgActcaaaagtccattgttttcttctgtgacggcacaggtggAccatt
gttatacaggca

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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