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CIS-REGULATION

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  1. Region 'REG00001191'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S816:16094-18001|Zicl

Short name

ZicL (-2kb)

Region ID

REG00001191

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Weiyang Shi (2017-03-23)

Annotator

Marion Gueroult-Bellone (2017-03-23)

Curator

Delphine Dauga (2017-06-12)

View position in WashU

Description

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.S816.1 (ZIC2; ZIC3; ZIC4)

Construct(s) made to test this region

CONS00001177: p.Cirobu.REG.KH2012.S816:16094-18001>GFP

CONS00001207: p.Cirobu.REG.KH2012.S816:16094-18001>YFP:caax

CONS00001206: p.Cirobu.REG.KH2012.S816:16094-18001>mCherry:caax

CONS00001208: p.Cirobu.REG.KH2012.S816:16094-18001>LacZ

CONS00001178: p.Cirobu.REG.KH2012.S816:16094-18001>CFP

Show in situ data
Overview of functional TF binding sites in this region

16,09418,001

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [16,094 / 18,001] on scaffold KhS816

1,9 kb of the ZicL 5prime flanking regulatory sequence, which was amplified from genomic DNA using the following primers: forward CATATTGTAAGGTGGAGATG and reverse TGCATAGGGACGATCAACC. This region drives expression in the progenitors of the notochord and nerve cord.

Show sequence

Length: 1,908

tgcatagggacgatcaaccattacattagaatacatattgtaagatgatgaacttctctg
gtatgctgactgccgtaaactaatgacatcacgctgattgaccttggtatttgtacctgt
cgcgtgctacgccttcctttcgtaacacaatgacttaattatcttctttatctttattta
tttgacttccgatcgcccgtcgctttcccatgcgcacgtggttcgatccccccacctccg
atatttgcctcgcgaaggtaattatcgtgtcgatcgcgcgtctcacgctacttttggcac
gctttcgctcgtgcctgtcgacgcaaggtttacgtatggcactttctctacgtgatgtca
acaacaagtcaacaaatttactcatcgtagagaataattgcaaaagtaaaaaatgttttt
cgttcgttcgaaaataatacaacacattgtagggtgggggaggtgtgacccttttgattc
tatccccattggtaacaagcaaagtacattcaaacaattatcaaccgtcttctcacgact
ttgatagacctatgttaatttttttatacatggttagaatattgggatattatgtgctaa
aggggtcctgtctccccccaccctactatataaaatttcaagcaagattgaagttaagta
tttacgcaatattgtgtcccaagcgttgcttgaaaattcatggaatttaaatttcagaaa
ctcattgcgtaattaaccaagtagaattacgataattacacgtattgttaaacttcctct
ataaaaacccgtcatcatgacgtcatcactcgacgactagttcgacgtcaaaacgattta
gtcgaataaaaaaataatccgtatctggaatactcgattttctgttcaatttttattatg
ttcttgtttgcaaagtaagtttgataggtaatttttgatcagcaaatttaataaaaacaa
gaaaactgcgctctaataaatatatattataacttgattctaaaacaagatacgtaattg
cacattataaaacactataatcaaatattaaactgcaaacttggtcattgtataagttat
agcaatacaatttatgttccatttatttgggctgaatcgtttatgtcatttccgttcact
aaactataatcctttataatctttacacacgctacaatgactcgagacaaaccgatttat
aaataatgatgacgtaacaattaacaaagggtctggaatgttgaattcgccataacttgc
tgttatcgagtttattataagattatttttgaaatttaaaaggtttagtattaagtcagg
actcaggagtatgaaaatgataactgacagataataataatttttatcaggtcggtaagc
tagcattactatattggaaaaacctaaagggaataaacaagtaaaacacgcatttttttt
tattaaaataattgggaatcggtaatttaaatctgaaaaattactgttaattatgatctg
gtaataactttcaaaatattaatagacgttttctatgtatcttacttgtgtaaatatttt
ttacaacgtgtaaatttgttgttgacatcacgtagagaaagtgccatacgtaaaccttgc
gtcgacaggcacgagcgaaagcgtgccaaaagtagcgtgagacgcgcgatcgacacgata
attaccttcgcgaggcaaatatcggaggtggggggatcgaaccacgtgcgcatgggaaag
cgacgggcgatcggaagtcaaataaataaagataaagaagataattaggtcattgtgtta
cgaaaggaaggcgtagcacgcgacaggtacaaataccaaggtcaatcagcgtgatgtcat
tagttcacggcagtcaggataccgaagaatattccatcttacaatatg

Reference(s)

Gainous et al. (2015). Diverse ETS transcription factors mediate FGF signaling in the Ciona anterior neural plate. Dev. Biol. 399(2):218-25

Shi et al. (2008). Ephrin signaling establishes asymmetric cell fates in an endomesoderm lineage of the Ciona embryo. Development 135(5):931-40

Wagner et al. (2012). FGF signaling establishes the anterior border of the Ciona neural tube. Development 139(13):2351-9

Abitua et al. (2015). The pre-vertebrate origins of neurogenic placodes. Nature 524(7566):462-5

Imai et al. (2009). Gene regulatory networks underlying the compartmentalization of the Ciona central nervous system. Development 136(2):285-93

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