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CIS-REGULATION

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  1. Region 'REG00001190'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S544:4240-5239|Dmrt

Short name

Dmrt 1kb upstream

Region ID

REG00001190

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Eileen Wagner (2017-03-23)

Annotator

Marion Gueroult-Bellone (2017-05-26)

Curator

Delphine Dauga (2017-06-12)

View position in WashU

Description

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.S544.3 (DMRT1; DMRTA1; DMRTA2)

Construct(s) made to test this region

CONS00001203: p.Cirobu.REG.KH2012.S544:4240-5239>H2B:CFP

CONS00001176: p.Cirobu.REG.KH2012.S544:4240-5239>GFP

CONS00001205: p.Cirobu.REG.KH2012.S544:4240-5239>H2B:YFP

CONS00001196: p.Cirobu.REG.KH2012.S544:4240-5239>mCherry-caax

CONS00001202: p.Cirobu.REG.KH2012.S544:4240-5239>H2B:mCherry

CONS00001201: p.Cirobu.REG.KH2012.S544:4240-5239>YFP:caax

CONS00001204: p.Cirobu.REG.KH2012.S544:4240-5239>LacZ

Show in situ data
Overview of functional TF binding sites in this region

4,2405,239

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,240 / 5,239] on scaffold KhS544

The DMRT enhancer was amplified from Ciona intestinalis genomic DNA with the following primers : DMRT-F (5prime;-GAAGTACTGCAGTAGTAGGGTGGAGGAAGATGGGAC) and DMRT NR (5prime;-AAAGCGGCCGCCATGCCAGTTAAACGAACTGTTTG); it drives expression throughout the anterior neural plate and adjacent anterior ectoderm

Show sequence

Length: 1,000

tagtagggtggaggaagatgggacacctttggcacataatatccaaatatccttaccccg
ttttacacaattaacagcggtatatgggagtcgtaagaattccgttttataattatttgc
atgttctttgtttgctactaaattggacgacaaaatgtagtcaaaaggtgtctaatcttc
ccctaccctactatacgacttggggcatagttataaagttattccgtttcatttcttggc
gttaacccgacgatcagtaattaacgatattcttcataaataaataatgcgagaactgca
acaggactagggaagttacttaagcgagcatgtgattagaaattgccactgaagcacttt
aaaatgccgccaaaacacatacaccttcatgacacattgttttacagaatctcaatttaa
aacccgtcgacaattatcggatttccgccgcagagtcacaggaagttgttttgcttaata
atacttgttttagataagctgttatgttgctcaatgaataacaataatgtttacgttggt
tgttgtggcaatgtacattgtaacttccacaaacctctcagagggcaaacacattaagca
aactgaatttgccaagtacttcgggaagtcactgataagcggacaatacgaagcgatcac
agggaattgtcgcacgccagaatgtaaacaaaaacatcgcctgtgcaccgcgcgatcgaa
gacacacagcgctgactaattgaccaacacaaaagcgaggaaagaaaaaaaagcgaaaac
gagaacgggatattaattgtacacgattgtgacgtaaattcgaggcattttttcttccga
aatcgagcggtttaaatacctcgcgtctttattaggtacaatcatattgccttgatacct
tgcttagaacagtgaatacatataaatcgtattaaaagctttagagtgttaaaccaaaga
gttacagaaattatagcaaacagttcgtttaactggcatg

Reference(s)

Gainous et al. (2015). Diverse ETS transcription factors mediate FGF signaling in the Ciona anterior neural plate. Dev. Biol. 399(2):218-25

Wagner et al. (2012). FGF signaling establishes the anterior border of the Ciona neural tube. Development 139(13):2351-9

Abitua et al. (2015). The pre-vertebrate origins of neurogenic placodes. Nature 524(7566):462-5

Wagner et al. (2014). Islet is a key determinant of ascidian palp morphogenesis. Development 141(15):3084-92

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