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CIS-REGULATION

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  1. Molecular Tool 'FGF8/17/18>CaEph3 (Stolfi 2011)' > 
  2. Transcript 'Moocci.CG.ELv1_2.S59...' > 
  3. Molecular Tool 'Dll-b>Bmp2/4 (Imai 2012)' > 
  4. Molecular Tool 'Hox2-MO (Ikuta 2010)' > 
  5. Region 'REG00001162'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA_AP2-1,2deletion

Short name

Ci-cdc25(-2109/+3) µGATA_AP2-1,2deletion

Region ID

REG00001162

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Yosuke Ogura (2017-02-17)

Annotator

Marion Gueroult-Bellone (2017-02-17)

Curator

Delphine Dauga (2017-05-30)

View position in WashU

Description

This sequence contains the cis-regulatory module CRM2 and CRM1 (as defined by Ogura and Sasakura, 2016). In this variant, both GATA binding sites of CRM1 were mutated (replaced by AATA), and both AP-2 binding sites of CRM2 were deleted.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA by deletion

Regulated gene(s)

KH2012:KH.C5.12 (CDC25A; CDC25B; CDC25C)

Construct(s) made to test this region

CONS00001136: pSP.Cirobu.REG.KH2012.C5:4201966-4204077(µGATA_AP2-1 ,2deletion):prCirobu.bpForkhead>Kaede

Show in situ data
Overview of functional TF binding sites in this region

4,201,9664,204,077

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 SOX n/a ttcaacg [4,203,985 / 4,203,992] This binding site is one of the 3 Sox sites of the cis-regulatory module 2 of Cdc25 (KH.C5.12). When all of them are deleted, this CRM is no longer able to drive expression in the epidermis.
2 SOX n/a cattgtt [4,204,030 / 4,204,037] This binding site is one of the 3 Sox sites of the cis-regulatory module 2 of Cdc25 (KH.C5.12). When all of them are deleted, this CRM is no longer able to drive expression in the epidermis.
3 SOX n/a cattgtt [4,204,062 / 4,204,069] This binding site is one of the 3 Sox sites of the cis-regulatory module 2 of Cdc25 (KH.C5.12). When all of them are deleted, this CRM is no longer able to drive expression in the epidermis.

Sequence

Aniseed Coordinates: [4,201,966 / 4,204,077] on scaffold KhC5

In this sequences, two GATA binding sites were mutated and both AP-2 sites were deleted.

Show sequence

Length: 2,112


catattaaagacaggtgctgaagattgaccacaagtgtataacacccgctagcttacaac
agagtaagctctcaagataccgtatgtctaaaacgcaattagctacttcggatgtcaaat
aacctgaaattctgcaaaagaaaacagtttgcttactcgatcggcgaagatactctgcta
atccacgagatatcggcttatgacaaacgcccataagttacatcgccgatatgacccaat
tccatcggcaatacgaccactatgttattgcggtctttactaagtcataatccaacttta
taacgcttgcgtcgcagaacaactgttgcaataagctccaatggccacaccatatgttac
gctcgaggtataggaggccttttgagtatatacccgacattctaatactactcactcaat
ataatacacaatacaacgtatgtatgtatgctgcatggtactctcttaatcaacacaata
aaatgacatatccaaaaccccaataatactatagccagctaactagtatacgactcttag
attctagttattgtgcaatgaaccgtctagtatcgtcagttaaacaaccatgtaatagca
tagtctctttctctcagtgctcgaggagcattccactttagttttaacgcactacaaact
actatacatacgcaatttctttcagttaccaatacgctatagtcgctatataacgtggta
gcctcacgctacagttatgtgctttagatgtgaatacatccgcctgtcgatactggccac
ttgtcctatatacattggatacggcttatcggcacaaagtaaacaataacaatgcgtagt
atgtggtctgcaatgcgctgctctgggtatactggaacggtactacattgttgtacagta
atgataaagttttacgtagaccagtaactacctagaggtagctgaataggtgaatactgt
ttgactcttatgcttctgtctagtaatatataagagccgaagtctgtctcgctgcttaat
acgcgctcaaactgaaatcccaggcaagatgtctacgattaggaagttgagaaaaaaggc
ccaagagctatgagctaattcccttgcattgtccggtttggacgctaattgatattctga
aggcagctcatgtaaactggtttacgaagcatcttacaatccggccaagctgggacacag
cgtgcgagtgactacctttacctgtgacgcgattaccaagcgagagattaacctttttcc
tttggcggtgagtcgaaaaggaaacataccgctgggctctcgctcggtagcgagtttgct
gctggcaagtaaatcctacgaccaacaatactagcgcaccttggcccggctggatgcagt
caggatgtctcggttggctgtttgtacgctatgcgggcactatggatgcacgcaagtgga
ttaacgggtgggattcttgcttaatatatccgaagttgggaactagttacggattaggca
gcgagtcggttctaatttttatagaaaagtttgctccgcggagatatttacgagaagcac
cacatatatattgacgacgtatttttgcttactactactgtcctactaacgttgcacgaa
aaacacacaactagtcttcctttaactatgcaggcagaacatgagtgaatactagattag
ttttaattaacgaatcggtcactatcctgcactgttgcatattctccaattacatggaat
attgctaataatgcatgtataccgaatcgatctactaacaaattgaactcagctggttgt
gcgctgcatgtgcagttgaatgaatggtgccctgcatcgaaccgcagctgctattttaag
agtgctggtgcagtcgcctttttctcgctttactacaggttcagtgtgttttggtcgtcc
actgataagtagcgaaggccgaagcgtcgcggttcgggccccgaggcacgcactattcta
gcgtcataaataagagctggattcgttctaataggaccgttcaacgaattgcctctcctt
tcacct---------tcaaaagtccattgttttcttctgtgacggca---------catt
gttatacaggca

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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