Description

The 135pb region located upstream Gdf1/3-r TSS is sufficient for driving reporter expression specifically in the animal hemisphere. It contains 3 GATA binding sites.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C4.547 (BMP2; BMP4; GDF5)

Construct(s) made to test this region

n/a

Overview of functional TF binding sites in this region

1,881,8831,882,061

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	ZnF_(GATA)	n/a	gata	[1,881,927 / 1,881,931]	One of the three GATA binding sites located in the upstream enhancer of Gdf1/3-r (C4.547).
2	ZnF_(GATA)	n/a	tatc	[1,881,944 / 1,881,948]	One of the three GATAa binding sites located in the upstream enhancer of Gdf1/3-r (C4.547).
3	ZnF_(GATA)	n/a	tatc	[1,881,955 / 1,881,959]	One of the three GATAa binding sites located in the upstream enhancer of Gdf1/3-r (C4.547).

Sequence

Aniseed Coordinates: [1,881,883 / 1,882,061] on scaffold KhC4

Sequence retrieved thanks to the supporting information of the article (S7G Fig.) Cap letters correspond to bases located downstream Gdf1/3-r TSS (KH.C4.547.v1.A.nonSL6-1).

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045