- Region 'REG00001151'
Cis-regulatory Region
Name
Cirobu.REG.KH2012.C3:2806773-2807200_μGATA(x3)|Efna.d
Short name
Efna.d (-220)_μGATA(x3)
Region ID
REG00001151
Status
Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
inactive_region
Author
Izumi Oda-Ishii (2017-02-01)
Annotator
Marion Gueroult-Bellone (2017-09-03)
Curator
Marion Gueroult-Bellone (2018-03-13)
Mutating three GATA sites of Efna.d 419pb minimal enhancer abolishes its activity.
Modification of Cirobu.REG.KH2012.C3:2806773-2807200|Efna.d by substitution
- Cirobu.REG.KH2012.C3:2806773-2809874|Efna.d REG00001146 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C3:2806773-2807200|Efna.d REG00001147 [ regulatory_region (extended_promoter) ]
CONS00001120: p.Cirobu.REG.KH2012.C3:2806773-2807200_μGATA(x3)>GFP
2,806,7732,807,200
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | ZnF_(GATA) | n/a | ctatct | [2,807,059 / 2,807,065] | One of the 4 GATA4/5/6 (Gata.a) binding sites located on the 419pb upstream enhancer of KH.C3.716. When 3 or 4 of these TFBS are missing or mutated, the enhancer is inactive. |
Aniseed Coordinates: [2,806,773 / 2,807,200] on scaffold KhC3
Three of the four GATA located in the 419pb Efna.d minimal enhancer are mutated : TATC, TATC and GATA respectively replaced by GCGA, GCGA and TCGC (positions # 320-323, 351-354 and 395-398 of the above sequence).
Show sequence
Length: 428
TTTAGGATctgaaaagtaaacacatatttagataactgaaatactataagcctttacttc
taaaaatcaaactggttgacatttattcttactgagcttgtggtattataaaatacaaac
gattagacgctatcaacttatttaatcgaactgcgttgaattcggtctcacgaaattgta
agctgcttactctgcgaaccgagatacactgatttgcggtagagagtcgaccgctataaa
gccacacaaattacggacttcttctctttttttcggtcgctgttcgctatctctcgtctg
accaatcgagagtccagctgcgaataatatttatgctcgcactcgtccctgcgatgctta
ttagcttggcgcccacgatttgcagagtattggttcgcacggtattgtgtccgtgtgcaa
tattgcaa
