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CIS-REGULATION

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  1. Region 'REG00001150'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C3:2806773-2807000|Efna.d

Short name

Efna.d (-220)

Region ID

REG00001150

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Izumi Oda-Ishii (2017-02-01)

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

The 220pb region located upstream Efna.d TSS has no enhancer activity. Compared to the 419pb minimal enhancer, it lacks all four GATA binding sites.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C3:2806773-2807099|Efna.d by deletion

Regulated gene(s)

KH2012:KH.C3.716 (EFNA1; EFNA4; EFNA5)

Construct(s) made to test this region

CONS00001119: p.Cirobu.REG.KH2012.C3:2806773-2807000>GFP

Show in situ data
Overview of functional TF binding sites in this region

2,806,7732,807,000

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [2,806,773 / 2,807,000] on scaffold KhC3

Sequence retrieved via the tool BLAST of Aniseed, thanks to the supporting information of the article (S7 Fig.)

Show sequence

Length: 228

TTTAGGATctgaaaagtaaacacatatttagataactgaaatactataagcctttacttc
taaaaatcaaactggttgacatttattcttactgagcttgtggtattataaaatacaaac
gattagacgctatcaacttatttaatcgaactgcgttgaattcggtctcacgaaattgta
agctgcttactctgcgaaccgagatacactgatttgcggtagagagtc

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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