- Region 'REG00001149'
Cis-regulatory Region
Name
Cirobu.REG.KH2012.C3:2806773-2807099|Efna.d
Short name
Efna.d (-319)
Region ID
REG00001149
Status
Curated
Origin
natural region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(extended_promoter)
Author
Izumi Oda-Ishii (2017-02-01)
Annotator
Marion Gueroult-Bellone (2018-03-13)
Curator
Marion Gueroult-Bellone (2018-03-13)
The 319pb region located upstream Efna.d TSS drives reporter expression specifically in the animal hemisphere. Compared to the 419pb minimal enhancer, it lacks 2 GATA binding sites (only 2 left) and has a weak enhancer activity.
Modification of Cirobu.REG.KH2012.C3:2806773-2807141|Efna.d by deletion
- Cirobu.REG.KH2012.C3:2806773-2809874|Efna.d REG00001146 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2012.C3:2806773-2807200|Efna.d REG00001147 [ regulatory_region (extended_promoter) ]
2,806,7732,807,099
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
|---|---|---|---|---|---|
| 1 | ZnF_(GATA) | n/a | ctatct | [2,807,058 / 2,807,064] | One of the 4 GATA4/5/6 (Gata.a) binding sites located on the 419pb upstream enhancer of KH.C3.716. When 3 or 4 of these TFBS are missing or mutated, the enhancer is inactive. |
| 2 | ZnF_(GATA) | n/a | ttatca | [2,807,090 / 2,807,096] | One of the 4 GATA4/5/6 (Gata.a) binding sites located on the 419pb upstream enhancer of KH.C3.716. When 3 or 4 of these TFBS are missing or mutated, the enhancer is inactive. |
Aniseed Coordinates: [2,806,773 / 2,807,099] on scaffold KhC3
Sequence retrieved via the tool BLAST of Aniseed, thanks to the supporting information of the article (S7 Fig.)
Show sequence
Length: 327
TTTAGGATctgaaaagtaaacacatatttagataactgaaatactataagcctttacttc
taaaaatcaaactggttgacatttattcttactgagcttgtggtattataaaatacaaac
gattagacgctatcaacttatttaatcgaactgcgttgaattcggtctcacgaaattgta
agctgcttactctgcgaaccgagatacactgatttgcggtagagagtcgaccgctataaa
gccacacaaattacggacttcttctcttttttcggtcgctgttcgctatctctcgtctga
ccaatcgagagtccagcttatcataat
