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CIS-REGULATION

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  1. Region 'REG00001130'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C2.4475337-4475581_Tcf7 from Tbx6|Fgf9/16/20

Short name

n/a

Region ID

REG00001130

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Izumi Oda-Ishii (2017-01-31)

Annotator

Alexis Roberge (2017-01-31)

Curator

Delphine Dauga (2017-09-13)

View position in WashU

Description

Replacing the Tcf7 sites critical for expression of Fgf9/16/20 in the anterior and posterior vegetal cells with the proximal Tcf7 site critical for expression of Tbx6.b in the posterior vegetal cells did not affect specificity of the reporter gene expression Fig.5C in the article.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C2.4475337-4475581|Fgf9/16/20 by substitution

Regulated gene(s)

KH2012:KH.C2.125 (FGF16; FGF20; FGF9)

Construct(s) made to test this region

CONS00001325: p.Cirobu.REG.KH2012.C2.4475337-4475581_Tcf7 from Tbx6>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,475,3374,475,581

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,475,337 / 4,475,581] on scaffold KhC2

The two Tcf7 sites critical for expression of Fgf9/16/20 in the anterior and posterior vegetal cells are replaced by the proximal Tcf7 site critical for expression of Tbx6.b in the posterior vegetal cells. Modified sequences are shown in capital letters

Show sequence

Length: 245

TCACTTTGATCcaattaagccgccatatagataactgtgcgcactggtcgtctgcccatt
tttagatagccttcccagtgcttctgttctcgagattacgatcacaagcgtaagacgtca
agttcacGATCAAAGTGAagaatcgcgacccacacaagtcactgccgccccttcttcgtt
tctcgtcgtacacaaaagcagcgggaatcttgactcggagcatcactcttgaatcgccac
tcagc

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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