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CIS-REGULATION

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  1. Region 'REG00001123'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:4424417-4425395_μTcf7(a)|Wnttun5

Short name

Wnttun5 (-979pb)_μTcf7(a)

Region ID

REG00001123

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Izumi Oda-Ishii (2017-01-31)

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

Mutation of one of the two Tcf7 binding sites strongly reduces Wnttun5 enhancer activity which remains highly specific of the posterior vegetal hemisphere.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:4424417-4425395|Wnttun5 by substitution

Regulated gene(s)

KH2012:KH.C9.257 (WNT2; WNT4; WNT6)

Construct(s) made to test this region

CONS00001092: p.Cirobu.REG.KH2012.C9:4424417-4425395_μTcf7(a)>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,424,4174,425,395

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a caaa [4,425,387 / 4,425,391] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.

Sequence

Aniseed Coordinates: [4,424,417 / 4,425,395] on scaffold KhC9

One of the two Tcf7 bindong site was mutated : TAAAC replaced by AGGGT (bases #924-928 from the above sequence).

Show sequence

Length: 979


tgatacttctgaacctgattgacttaaagtactggctcatatagtaagtcgaagattgtc
cagcgcccctgtgctcaaaccttttctctgatgtcctgtggcgacgctggcgctggtgta
ggtgtgaattcgggaaaatctcgtaaactcgatgttatcgttcagtccatccagtttcgg
gcgtaagtagcgccctagtccgggttgttctcgcctctgcaagtgcgaactttcttctga
gttgatttagtttagcgacagtggctggaaaaattcgtgaacccagacctacaacctgct
gctgttaaaattagacgtttactttgctgttggttataggtacatattatcaaaacagcc
gtatagctttccaatcagaaacaaatgctgatcacatccgtatcaaatccgtcacgattt
tacgaactgagtaatatcgtttcgactgtggctgcctcttgtaatcacctctactctctg
cactgccatctgaggtagagctttaaatgggagaacggcaatcgccaacggcaaaacgaa
aatgtcttcaagtttttcgctagcgcctatggtgacccatcggttgtacctaattgtcga
tactgacgtagtttcaaaatacccctaactttcatatgacctccgccacttaaaagaggt
gatggacagaagtgtcacaggtgaagtttctgatcctaatatacccggacgagtctcggc
ctcccaataggtggtcggcggcgatcgcaatcgtaattgctcgattccaacccctgccca
gcactaggggcgaggaaaccgccaattatcaaatagatcagtcgcttgcgcatccccgta
tgcggagatcggccttgaaataacaaactgccgacaaaactcggcgggccaattaaagcc
cctgtagctcgcaaatgcagagaagggtaaacaagctgctcgcgtgtggacctgtaatta
cggcagcgatcaaacgacg

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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