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CIS-REGULATION

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  1. Region 'REG00001122'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:4424417-4425208|Wnttun5

Short name

Wnttun5 (-792pb)

Region ID

REG00001122

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Izumi Oda-Ishii (2017-01-31)

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

792pb upstream Wnttun5 TSS. This region has no enhancer activity and lacks two critical Tcf7 binding sites located upstream.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:4424417-4425395|Wnttun5 by deletion

Regulated gene(s)

KH2012:KH.C9.257 (WNT2; WNT4; WNT6)

Construct(s) made to test this region

CONS00001091: p.Cirobu.REG.KH2012.C9:4424417-4425208>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,424,4174,425,208

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,424,417 / 4,425,208] on scaffold KhC9

Sequence retrieved via the tool BLAST of Aniseed, thanks to the supporting information of the article (S5 Fig.)

Show sequence

Length: 792

tgatacttctgaacctgattgacttaaagtactggctcatatagtaagtcgaagattgtc
cagcgcccctgtgctcaaaccttttctctgatgtcctgtggcgacgctggcgctggtgta
ggtgtgaattcgggaaaatctcgtaaactcgatgttatcgttcagtccatccagtttcgg
gcgtaagtagcgccctagtccgggttgttctcgcctctgcaagtgcgaactttcttctga
gttgatttagtttagcgacagtggctggaaaaattcgtgaacccagacctacaacctgct
gctgttaaaattagacgtttactttgctgttggttataggtacatattatcaaaacagcc
gtatagctttccaatcagaaacaaatgctgatcacatccgtatcaaatccgtcacgattt
tacgaactgagtaatatcgtttcgactgtggctgcctcttgtaatcacctctactctctg
cactgccatctgaggtagagctttaaatgggagaacggcaatcgccaacggcaaaacgaa
aatgtcttcaagtttttcgctagcgcctatggtgacccatcggttgtacctaattgtcga
tactgacgtagtttcaaaatacccctaactttcatatgacctccgccacttaaaagaggt
gatggacagaagtgtcacaggtgaagtttctgatcctaatatacccggacgagtctcggc
ctcccaataggtggtcggcggcgatcgcaatcgtaattgctcgattccaacccctgccca
gcactaggggcg

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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