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CIS-REGULATION

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  1. Region 'REG00001121'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C9:4424417-4425395|Wnttun5

Short name

Wnttun5 (-979pb)

Region ID

REG00001121

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Izumi Oda-Ishii (2017-01-31)

Annotator

Marion Gueroult-Bellone (2018-03-13)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

The 979pb sequence located upstream of Wnttun5 TSS drives reporter expression specifically in the posterior vegetal hemisphere. It contains two critical Tcf7 binding sites.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C9:4424417-4425816|Wnttun5 by deletion

Regulated gene(s)

KH2012:KH.C9.257 (WNT2; WNT4; WNT6)

Construct(s) made to test this region

CONS00001090: p.Cirobu.REG.KH2012.C9:4424417-4425395>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,424,4174,425,395

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a taaac [4,425,340 / 4,425,345] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.
2 TCF/LEF n/a caaa [4,425,387 / 4,425,391] One of the two Tcf7 binding sites located on the upstream enhancer of KH.C9.257 (Wnttun5). Mutating one of these TFBS strongly reduces enhancer activity, and when both are missing, the enhancer is no longer active.

Sequence

Aniseed Coordinates: [4,424,417 / 4,425,395] on scaffold KhC9

Sequence retrieved via the tool BLAST of Aniseed, thanks to the supporting information of the article (S5 Fig.)

Show sequence

Length: 979


tgatacttctgaacctgattgacttaaagtactggctcatatagtaagtcgaagattgtc
cagcgcccctgtgctcaaaccttttctctgatgtcctgtggcgacgctggcgctggtgta
ggtgtgaattcgggaaaatctcgtaaactcgatgttatcgttcagtccatccagtttcgg
gcgtaagtagcgccctagtccgggttgttctcgcctctgcaagtgcgaactttcttctga
gttgatttagtttagcgacagtggctggaaaaattcgtgaacccagacctacaacctgct
gctgttaaaattagacgtttactttgctgttggttataggtacatattatcaaaacagcc
gtatagctttccaatcagaaacaaatgctgatcacatccgtatcaaatccgtcacgattt
tacgaactgagtaatatcgtttcgactgtggctgcctcttgtaatcacctctactctctg
cactgccatctgaggtagagctttaaatgggagaacggcaatcgccaacggcaaaacgaa
aatgtcttcaagtttttcgctagcgcctatggtgacccatcggttgtacctaattgtcga
tactgacgtagtttcaaaatacccctaactttcatatgacctccgccacttaaaagaggt
gatggacagaagtgtcacaggtgaagtttctgatcctaatatacccggacgagtctcggc
ctcccaataggtggtcggcggcgatcgcaatcgtaattgctcgattccaacccctgccca
gcactaggggcgaggaaaccgccaattatcaaatagatcagtcgcttgcgcatccccgta
tgcggagatcggccttgaaataacaaactgccgacaaaactcggcgggccaattaaagcc
cctgtagctcgcaaatgcagagataaacaaacaagctgctcgcgtgtggacctgtaatta
cggcagcgatcaaacgacg

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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