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CIS-REGULATION

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  1. Region 'REG00001117'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C8:2621713-2622953_μTcf7(x5)|Foxd.b

Short name

Foxd.b (-1241pb)_μTcf7(x5)

Region ID

REG00001117

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Izumi Oda-Ishii (2017-01-31)

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

Mutation of five Tcf7 binding site of the 5' region of the 1.2kb Foxd.b enhancer. The mutation of these sites abolishes enhancer activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C8:2621713-2622953|Foxd.b by substitution

Regulated gene(s)

KH2012:KH.C8.396 (FOXD1; FOXD3; FOXD4)

Construct(s) made to test this region

CONS00001086: p.Cirobu.REG.KH2012.C8:2621713-2622953_μTcf7(x5)>GFP

Show in situ data
Overview of functional TF binding sites in this region

2,621,7132,622,953

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [2,621,713 / 2,622,953] on scaffold KhC8

Five Tcf7 binding sites are mutated : TTTG or TTTA core binding are replaced by non-consensus CCCCA. Positions of the mutated TFBS on the above sequence : 19-22, 68-71, 84-87, 139-142 and 171-174.

Show sequence

Length: 1,241

tttgtttactttttttaccccatttatgttaagtaaagtttaatttcattaaagatgtta
gttcgaccccaatcgtagcttagcccaactgatgtcgcgagtgattaggcgataagaagg
cgagaaatatcatcgatgcccattcccgatatttggcggcgtttgtggcgcccaaaagag
gcgagttcgagtcgcgagtctcgatcttttcatctttatctcggttgaattgatttgtcg
tgaataaattgggaagttgtgtcgattggaacatcagttgcaagaatgagttgaacagtt
ggtccaattcaaacgttacttgcagtaaaacttgtgtggacaagttataacttaatacga
ggtgtatataaagtcgttagaattttacgaatcagttgacaaatataaataaaggtacag
gtagttttgttgaacattgcgacagtctgactgtgttttcagtttcgctaaatttctcgt
acggtaaaattgtttactaaaaaataagggcgtacgtaaatcaattctgggttgggtagc
tttaagcattacaataacgtacatattgcctgtttacaaatatagcagttatatttaaat
atatactaagaataaaggtgcagctcattcaaatttaatcgctttaccgattctctcaat
taagaataggagaaaacgcgtatttagataacatttaacgggaaacgactacagtcctaa
tattggaactacagacgttcaataaactcgttagcttgacatttatttcaatactgtttg
tgttacgtcacgggtatttttgagtcattattttaacgattggtcggattatttgaaaac
ctttaccacagtgtgttgttagaaaatagacgccatttttaccagcggaaccagcatttt
gccaaattcaaatttaataatttttttcagttgttttcaacattttgggacacttctcaa
aaaagcgaaatgttttttatgaaactcagtttttaaaaacagcgaaaacacgcatatttt
tgaacgcaaccattaatcgattaagttggacgtttttcttgtaaatgataaattaattag
ccttcttcttttgcttagaaaacattgtaatataatcttttgaaaaatctgttgctgatc
aacggaattataaacgagatctaggattcgaagaaaaaagcggttgctgggagaattgaa
gaagtggatttgaataaattgtcgcgttttgcaaagtgctc

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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