Login
Help

CIS-REGULATION

Submit your Data

  1. Region 'REG00001113'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S654:26922-27123_μTcf7|Tbx6.b

Short name

Tbx6.b (-189)_μTcf7

Region ID

REG00001113

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Izumi Oda-Ishii (2017-01-30)

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Delphine Dauga (2017-03-07)

View position in WashU

Description

Mutation of the two putative Tcf7-binding sites of Tbx6.b minimal enhancer abolishes its enhancer activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.S654:26922-27123|Tbx6.b by substitution

Regulated gene(s)

KH2012:KH.S654.3 (TBX2; TBX4; TBX6)

Construct(s) made to test this region

CONS00001081: p.Cirobu.REG.KH2012.S654:26922-27123_μTcf7>GFP

Show in situ data
Overview of functional TF binding sites in this region

26,92227,123

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [26,922 / 27,123] on scaffold KhS654

Both Tcf7 binding sites of Tbx6.b minimal enhancer where mutated : AAAAC and CTTTG resp. remplaced by GGGGT and TCCCA (bases #59-63 and 154-159 of the above sequence).

Show sequence

Length: 202

cggaatgaggtactgtgaaactaaacccgttttatagcgagactcgttttcgatgccatc
ccaatcttaacgagtcattaacaaggacaactgcttcctgataagcctccgaactctcct
gctgattcaagtctagcgcatgcagaacactccggggttcagtgtgacgcataccgtctt
gcatgtggcgcgttggtttgat

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Back to Top