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CIS-REGULATION

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  1. Region 'REG00001111'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S654:26922-27123|Tbx6.b

Short name

Tbx6.b (-189)

Region ID

REG00001111

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Izumi Oda-Ishii ()

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

189b region located upstream Tbx6.b TSS. It is the smallest region able to drive gene expression in posterior vegetal hemisphere domain.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.S654:26922-27180|Tbx6.b by deletion

Regulated gene(s)

KH2012:KH.S654.3 (TBX2; TBX4; TBX6)

Construct(s) made to test this region

CONS00001079: p.Cirobu.REG.KH2012.S654:26922-27123>GFP

CONS00001312: p.(Cirobu.REG.KH2012.S654:26922-27123)x4>GFP

Show in situ data
Overview of functional TF binding sites in this region

26,92227,123

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a ctttg [26,980 / 26,985] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.
2 TCF/LEF n/a aaaac [27,075 / 27,080] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.

Sequence

Aniseed Coordinates: [26,922 / 27,123] on scaffold KhS654

Sequence retrieved via the tool BLAST of Aniseed, thanks to the supporting information of the article (S5 Fig.)

Show sequence

Length: 202


cggaatgaggtactgtgaaactaaacccgttttatagcgagactcgttttcgatgccact
ttgatcttaacgagtcattaacaaggacaactgcttcctgataagcctccgaactctcct
gctgattcaagtctagcgcatgcagaacactccaaaactcagtgtgacgcataccgtctt
gcatgtggcgcgttggtttgat

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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