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CIS-REGULATION

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  1. Region 'REG00001110'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S654:26922-27180|Tbx6.b

Short name

Tbx6.b (-246)

Region ID

REG00001110

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Izumi Oda-Ishii ()

Annotator

Marion Gueroult-Bellone (2017-09-03)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

246pb region located upstream Tbx6b TSS. It drives gene expression in posterior vegetal hemisphere domain.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.S654:26922-27386|Tbx6.b by deletion

Regulated gene(s)

KH2012:KH.S654.3 (TBX2; TBX4; TBX6)

Construct(s) made to test this region

CONS00001082: p.Cirobu.REG.KH2012.S654:26922-27180>GFP

Show in situ data
Overview of functional TF binding sites in this region

26,92227,180

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a ctttg [26,980 / 26,985] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.
2 TCF/LEF n/a aaaac [27,075 / 27,080] One of the 2 Tcf7 binding sites located on the 189pb upstream enhancer of KH.S654.3 (Tbx6.b). When 1 or both of these TFBS are missing or mutated, the enhancer is inactive.

Sequence

Aniseed Coordinates: [26,922 / 27,180] on scaffold KhS654

Sequence retrieved via the tool BLAST of Aniseed, thanks to the supporting information of the article (S5 Fig.)

Show sequence

Length: 259


cggaatgaggtactgtgaaactaaacccgttttatagcgagactcgttttcgatgccact
ttgatcttaacgagtcattaacaaggacaactgcttcctgataagcctccgaactctcct
gctgattcaagtctagcgcatgcagaacactccaaaactcagtgtgacgcataccgtctt
gcatgtggcgcgttggtttgatgaaatcgctttaaaaatgtgtttgataatttcattatt
atggtttgatgtagaatag

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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