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CIS-REGULATION

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  1. EST 'AV674167' > 
  2. EST 'JG341348' > 
  3. EST 'BW078075' > 
  4. EST 'AV988212' > 
  5. Region 'REG00001104'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C5:4201966-4203978|cdc25_µGATA BS1

Short name

Ci-cdc25(-2010/+3)_µGATA BS1

Region ID

REG00001104

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Yosuke Ogura (2017-01-30)

Annotator

Marion Gueroult-Bellone (2017-01-30)

Curator

Delphine Dauga (2017-05-30)

View position in WashU

Description

This sequence contains the short (27pb) cis-regulatory module CRM1 containing two GATA binding sites (as defined by Ogura and Sasakura, 2016). In this variant, the first GATA site was mutated (replaced by AATA). Sequence of CRM1-GATA BS1 mutated : tatcagaacgaatccagctcttattta

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C5:4201966-4203978 by substitution

Regulated gene(s)

KH2012:KH.C5.12 (CDC25A; CDC25B; CDC25C)

Construct(s) made to test this region

CONS00001072: pSP.Cirobu.REG.KH2012.C5:4201966-4203978(µGATA BS1):prCirobu.bpForkhead>Kaede

Show in situ data
Overview of functional TF binding sites in this region

4,201,9664,203,978

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 ZnF_(GATA) n/a gata [4,203,975 / 4,203,979] This binding site is one of the two GATA binding sites of the CRM1 defined by Ogura et al., 2016. Its mutation does not strongly affect its enhancer activity. When both of them are mutated, this CRM is no longer able to drive expression in the epidermis.

Sequence

Aniseed Coordinates: [4,201,966 / 4,203,978] on scaffold KhC5

The first GATA (located 21pb from the 5' of the above sequence) site was mutated : GATA was replaced by AATA.

Show sequence

Length: 2,013


catattaaagacaggtgctgaagattgaccacaagtgtataacacccgctagcttacaac
agagtaagctctcaagataccgtatgtctaaaacgcaattagctacttcggatgtcaaat
aacctgaaattctgcaaaagaaaacagtttgcttactcgatcggcgaagatactctgcta
atccacgagatatcggcttatgacaaacgcccataagttacatcgccgatatgacccaat
tccatcggcaatacgaccactatgttattgcggtctttactaagtcataatccaacttta
taacgcttgcgtcgcagaacaactgttgcaataagctccaatggccacaccatatgttac
gctcgaggtataggaggccttttgagtatatacccgacattctaatactactcactcaat
ataatacacaatacaacgtatgtatgtatgctgcatggtactctcttaatcaacacaata
aaatgacatatccaaaaccccaataatactatagccagctaactagtatacgactcttag
attctagttattgtgcaatgaaccgtctagtatcgtcagttaaacaaccatgtaatagca
tagtctctttctctcagtgctcgaggagcattccactttagttttaacgcactacaaact
actatacatacgcaatttctttcagttaccaatacgctatagtcgctatataacgtggta
gcctcacgctacagttatgtgctttagatgtgaatacatccgcctgtcgatactggccac
ttgtcctatatacattggatacggcttatcggcacaaagtaaacaataacaatgcgtagt
atgtggtctgcaatgcgctgctctgggtatactggaacggtactacattgttgtacagta
atgataaagttttacgtagaccagtaactacctagaggtagctgaataggtgaatactgt
ttgactcttatgcttctgtctagtaatatataagagccgaagtctgtctcgctgcttaat
acgcgctcaaactgaaatcccaggcaagatgtctacgattaggaagttgagaaaaaaggc
ccaagagctatgagctaattcccttgcattgtccggtttggacgctaattgatattctga
aggcagctcatgtaaactggtttacgaagcatcttacaatccggccaagctgggacacag
cgtgcgagtgactacctttacctgtgacgcgattaccaagcgagagattaacctttttcc
tttggcggtgagtcgaaaaggaaacataccgctgggctctcgctcggtagcgagtttgct
gctggcaagtaaatcctacgaccaacaatactagcgcaccttggcccggctggatgcagt
caggatgtctcggttggctgtttgtacgctatgcgggcactatggatgcacgcaagtgga
ttaacgggtgggattcttgcttaatatatccgaagttgggaactagttacggattaggca
gcgagtcggttctaatttttatagaaaagtttgctccgcggagatatttacgagaagcac
cacatatatattgacgacgtatttttgcttactactactgtcctactaacgttgcacgaa
aaacacacaactagtcttcctttaactatgcaggcagaacatgagtgaatactagattag
ttttaattaacgaatcggtcactatcctgcactgttgcatattctccaattacatggaat
attgctaataatgcatgtataccgaatcgatctactaacaaattgaactcagctggttgt
gcgctgcatgtgcagttgaatgaatggtgccctgcatcgaaccgcagctgctattttaag
agtgctggtgcagtcgcctttttctcgctttactacaggttcagtgtgttttggtcgtcc
actgataagtagcgaaggccgaagcgtcgcggttcgggccccgaggcacgcactattcta
gcgtcataaataagagctggattcgttctgata

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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