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CIS-REGULATION

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  1. Region 'REG00000380' > 
  2. Region 'REG00001103'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C5:4201966-4203978

Short name

Ci-cdc25(-2010/+3)

Region ID

REG00001103

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Yosuke Ogura (2017-01-30)

Annotator

Marion Gueroult-Bellone (2017-01-30)

Curator

Delphine Dauga (2017-05-24)

View position in WashU

Description

This sequence is part of the deletion analysis of the Ci-cdc25 upstream region. It contains the cis-regulatory module CRM1, but lacks CRM2 (as defined by Ogura and Sasakura, 2016). Region called -2010 in the article.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C5:4201966-4204077 by deletion

Regulated gene(s)

KH2012:KH.C5.12 (CDC25A; CDC25B; CDC25C)

Construct(s) made to test this region

CONS00001071: pSP.Cirobu.REG.KH2012.C5:4201966-4203978:prCirobu.bpForkhead>Kaede

Show in situ data
Overview of functional TF binding sites in this region

4,201,9664,203,978

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 ZnF_(GATA) n/a gata [4,203,954 / 4,203,958] This binding site is one of the two GATA binding sites of the CRM1 defined by Ogura et al., 2016. When it is mutated, the enhancer activity is strongly reduced. When both of them are mutated, this CRM is no longer able to drive expression in the epidermis.
2 ZnF_(GATA) n/a gata [4,203,975 / 4,203,979] This binding site is one of the two GATA binding sites of the CRM1 defined by Ogura et al., 2016. When both of them are mutated, this CRM is no longer able to drive expression in the epidermis.

Sequence

Aniseed Coordinates: [4,201,966 / 4,203,978] on scaffold KhC5

2kpb region located upstream the start codon of cdc25 (KHC5.12).

Show sequence

Length: 2,013


catattaaagacaggtgctgaagattgaccacaagtgtataacacccgctagcttacaac
agagtaagctctcaagataccgtatgtctaaaacgcaattagctacttcggatgtcaaat
aacctgaaattctgcaaaagaaaacagtttgcttactcgatcggcgaagatactctgcta
atccacgagatatcggcttatgacaaacgcccataagttacatcgccgatatgacccaat
tccatcggcaatacgaccactatgttattgcggtctttactaagtcataatccaacttta
taacgcttgcgtcgcagaacaactgttgcaataagctccaatggccacaccatatgttac
gctcgaggtataggaggccttttgagtatatacccgacattctaatactactcactcaat
ataatacacaatacaacgtatgtatgtatgctgcatggtactctcttaatcaacacaata
aaatgacatatccaaaaccccaataatactatagccagctaactagtatacgactcttag
attctagttattgtgcaatgaaccgtctagtatcgtcagttaaacaaccatgtaatagca
tagtctctttctctcagtgctcgaggagcattccactttagttttaacgcactacaaact
actatacatacgcaatttctttcagttaccaatacgctatagtcgctatataacgtggta
gcctcacgctacagttatgtgctttagatgtgaatacatccgcctgtcgatactggccac
ttgtcctatatacattggatacggcttatcggcacaaagtaaacaataacaatgcgtagt
atgtggtctgcaatgcgctgctctgggtatactggaacggtactacattgttgtacagta
atgataaagttttacgtagaccagtaactacctagaggtagctgaataggtgaatactgt
ttgactcttatgcttctgtctagtaatatataagagccgaagtctgtctcgctgcttaat
acgcgctcaaactgaaatcccaggcaagatgtctacgattaggaagttgagaaaaaaggc
ccaagagctatgagctaattcccttgcattgtccggtttggacgctaattgatattctga
aggcagctcatgtaaactggtttacgaagcatcttacaatccggccaagctgggacacag
cgtgcgagtgactacctttacctgtgacgcgattaccaagcgagagattaacctttttcc
tttggcggtgagtcgaaaaggaaacataccgctgggctctcgctcggtagcgagtttgct
gctggcaagtaaatcctacgaccaacaatactagcgcaccttggcccggctggatgcagt
caggatgtctcggttggctgtttgtacgctatgcgggcactatggatgcacgcaagtgga
ttaacgggtgggattcttgcttaatatatccgaagttgggaactagttacggattaggca
gcgagtcggttctaatttttatagaaaagtttgctccgcggagatatttacgagaagcac
cacatatatattgacgacgtatttttgcttactactactgtcctactaacgttgcacgaa
aaacacacaactagtcttcctttaactatgcaggcagaacatgagtgaatactagattag
ttttaattaacgaatcggtcactatcctgcactgttgcatattctccaattacatggaat
attgctaataatgcatgtataccgaatcgatctactaacaaattgaactcagctggttgt
gcgctgcatgtgcagttgaatgaatggtgccctgcatcgaaccgcagctgctattttaag
agtgctggtgcagtcgcctttttctcgctttactacaggttcagtgtgttttggtcgtcc
actgataagtagcgaaggccgaagcgtcgcggttcgggccccgaggcacgcactattcta
gcgtcatagataagagctggattcgttctgata

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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