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CIS-REGULATION

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  1. Region 'REG00001091'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C2.4475337-4475581_μTcf7(c)|Fgf9/16/20

Short name

Fgf9/16/20 (-219pb)_μTcf7(c)

Region ID

REG00001091

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Izumi Oda-Ishii (2017-01-17)

Annotator

Marion Gueroult-Bellone (2018-03-13)

Curator

Marion Gueroult-Bellone (2018-03-13)

View position in WashU

Description

Mutation of the more 3' Tcf7 binding site (called Tcf7(c) in the article). This mutation has little effect on the enhancer activity : it is still able to drive expression of the reporter gene specifically in the entire vegetal hemisphere domain.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C2.4475337-4475581|Fgf9/16/20 by substitution

Regulated gene(s)

KH2012:KH.C2.125 (FGF16; FGF20; FGF9)

Construct(s) made to test this region

CONS00001062: p.Cirobu.REG.KH2012.C2.4475337-4475581_μTcf7(c)>GFP

Show in situ data
Overview of functional TF binding sites in this region

4,475,3374,475,581

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 TCF/LEF n/a ttta [4,475,341 / 4,475,345] First of the 3 Tcf7 binding site located in FGF9/16/20 enhancer. Its mutation alone does not affect a lot the 219pb enhancer activity, but when both first and second Tcf7 binding sites are mutated, the enhancer is inactivated.
2 TCF/LEF n/a aaag [4,475,468 / 4,475,472] Second of the 3 Tcf7 binding site located in FGF9/16/20 enhancer. Its mutation alone does nearly anihilates the 219pb enhancer activity, and when associated to the mutation of another Tcf7 binding site, the enhancer is inactivated.

Sequence

Aniseed Coordinates: [4,475,337 / 4,475,581] on scaffold KhC2

The third Tcf7 binding site (TTTC) was replaced by CCCT (bases #179 to 182 of this sequence).

Show sequence

Length: 245


gttgtttacagcaattaagccgccatatagataactgtgcgcactggtcgtctgcccatt
tttagatagccttcccagtgcttctgttctcgagattacgatcacaagcgtaagacgtca
agttcaccgacaaagataagaatcgcgacccacacaagtcactgccgccccttcttcgcc
cttcgtcgtacacaaaagcagcgggaatcttgactcggaGCATCACTCTTGAATCGCCAC
TCAGC

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

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