Aniseed - Cis-regulation

Region 'REG00000203' >
Region 'REG00001027'

Cis-regulatory Region

Name

Cirobu.REG.KH2013:KH.C6.1849423-1851424

Short name

Ci-Fz4(-1964/+38)

Region ID

REG00001027

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Fujiwara team (2015-08-29)

Annotator

Shigeki Fujiwara (2015-08-29)

Curator

Delphine Dauga (2018-04-17)

View position in WashU

Description

Hierarchy of Regulatory Regions

Cirobu.REG.KH2013:KH.C6.1849423-1851424 REG00001027 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KH2013:KH.C6.1849962-1851424 REG00001028 [ regulatory_region (extended_promoter) ]
  - Cirobu.REG.KH2013:KH.C6.1850093-1851424 REG00001029 [ regulatory_region (extended_promoter) ]
    - Cirobu.REG.KH2013:KH.C6.1850412-1851424 REG00001030 [ regulatory_region (extended_promoter) ]
      - Cirobu.REG.KH2013:KH.C6.1850603-1851424 REG00001031 [ inactive_region ]
        
        Cirobu.REG.KH2013:KH.C6.1851000-1851424 REG00001032 [ inactive_region ]

Regulated gene(s)

KH2012:KH.C6.162 (FZD10; FZD4; FZD9)

Construct(s) made to test this region

CONS00000996: pCirobu.REG.KH2013:KH.C6.1849423-1851424>NLSLacZ

Show in situ data

Overview of functional TF binding sites in this region

1,849,4231,851,424

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [1,849,423 / 1,851,424] on scaffold KhC6

The regulatory region contains 1964-bp 5' flanking region, 5' UTR and 2-bp coding region. The DNA fragment was fused in frame with the translated region of lacZ.

Show sequence

Length: 2,002

AGTGAACGTAAATCCGTTTACCATTAACCGAACTAATTTCTTAGCTTATCAGTTCAGGCA
TCGAGTCTTGCCCTCCCAATTCCTGGCCAAGACGCTCCAAAGGCTCAAGCAATTACGAGG
ATTAAGACCCTATGTGCGCAGTTTGTTTGGCGGATTACTTTTCGCCGGATTTTGTTTCAT
CTTTCTAGTCAACATGTAAAAAGTAGAAAGTAAAGCTTTGATGTTCGGCTAATAAGATGC
ATGCAGTGGACTGGAACGAGCTTTATAACCAAAGCAGTACTTAAACTAGATTCGACTCGT
CCGAGTGCTTGTATAGCAATAGGCGGCATTGCACTCCGGGTGTCTTGAGAATGAGAACGG
TTTATCGTAGTTTTGGCAGTTAACTCAGTGCAGCGAATGGTTGAGGTTTTGGCTTTAAAG
TAAACGAAAAAAATTAAAAATAAATGTCGGTATAGTTTTTAACAGTTTACATTCTATGTA
GAAAATTACTAAAAACTGTTTTTTAAATTTTAAAAAACGATTGTCGTTTCGCCGCTATTC
CCTTCTCTTCGTTGTTTCAATAACTTTGATCATCGCTACTGTAAATCATACAATGAGTTT
ATGTTATGTTTTATTGTTTTACACGTTGAAATAAGATTAATTTACACCATCTAAGTTTCT
CTGGCTTATTTATCTATCGTCATGACCCATAAATTGGTTATTACTGTCAAATAGATAGCG
TTTGATGTTACCAGTATTTTAGGTCGCTGCGGTATTTTCAACTGGTTAGTGGTGTTACAC
CAAATTCGCTTTTTCGCGAAAACTGCTTTGGTTACTTCACGGTTCGATGCGTAAACGTAA
ATTATGGCTCGTTACGTCACAATTGGCTTTGTTTACGTGGCCAAGCGGAAACGATGACGA
AATTATATGAAATTCATTTTCATTATTTTTTGTTAAAATTATAATTTTTCAGTGAAACTT
TGTAGCTTTATTACAGAATTCTATATATGTATCTTCACGGTTCTAACACAGCGTTGTTTA
TTTCTTAAATCGCGATTGGGGATTATGGGATATTATGTGCCAACGGTATCCATGTTACCC
AACAGTACTATATTAAATATTTTGATTTGATTTTGAGACTTTCTGCAAACTAAAGATTGC
TTATCGTTTCCGTTACTTGACGTCATCATTTTGTTTCGTAAACAATAGAGATTGTGACGC
CACAGATAACAATTGCAGAAACTCGCGACCGCGAATTGCATTATCTGATAATCACTTTCT
ATTATGACGTAACACTTGCAGATCGTAGAGAAAAGAGGACGAGCTGGTTCTATTTAAATA
CAAAGCAAGTCGAAAGTGTTGGCAGCCAGTGTATGAAGTAAGGATCAACTGTCTTATAGT
AGGTTAAGGTAAAATATTCCCATGTTTTTATTCTCTTTTCTACGGCGCGCGCATTTGCAC
CAATGCTAAGGATGGGAGCATACAAGTTTAGGTGTGAATAACATAATATATGGTGGGGTA
AGATGGAACACGTTTTCATTCTCTTATTTCGTCCTATTTGGTGGTAAACAAAGAATATTT
AAGGAACTACATAACCGTATCCTCACCACTGTTAAGATCGTTGTTATTTGTTAAAAACGC
GATTAGGAAATATGAGATATTATGTTCTAACGGTATCCATCTACCCGACCTACTACACGT
TCTCCAACCCAGTATGCCCTACTACAGTGCGTGAGATGATAAGACAGTAGGGCATACACC
TGGTAACAGCGGGTGTTTAGTAAAGATCAACCTTTTTCCAATGCACGTTGTCTTAGTACA
GGTTTATTTAGTTTTGCGTTTAGGAAATTAGTTTGCAAGTGAAAAGCGCGTTGTTGTTTT
AATGTATTTATTTTATCTTACTTTGAATTGAGAAAAGGGTTATTTGTTGCAGATTTGTGT
ATATACTTTGTTATGGCATATATTTAAAACTGTGTTTCCTACAGGTTGTTTGAGTGCTAA
GTAAAATTGTATAATTGGACAT

Reference(s)

Horikawa et al. (2013). Transcriptional regulation in the early ectodermal lineage of ascidian embryos. Dev. Growth Differ. 55(9):776-85