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CIS-REGULATION

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  1. Region 'REG00000890'

Cis-regulatory Region

Name

Ci-Pitx -603/-353

Short name

n/a

Region ID

REG00000890

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Lionel Christiaen (2011-08-10)

Annotator

Delphine Dauga (2011-08-10)

Curator

Delphine Dauga (2011-08-10)

View position in WashU

Description

Region from -3164 bp upstream the +1 to the first 55 bp of the exon 1 drives the entire Ci-Pitxa/b expression.

Region from -3164 bp to -2043 bp drives expression in stomodeal cells

Region from -2043 bp to -1776 bp drives expression in tail muscles

Region from -1776 bp to +55 pb drives ectopic expression in the mesenchyme.

Hierarchy of Regulatory Regions

Modification of Ci-Pitx -603/140 by deletion

Regulated gene(s)

KH2012:KH.L153.79 (PITX1; PITX2; PITX3)

Construct(s) made to test this region

CONS00000856: pCi-Pitx -603/-353::LacZ

Show in situ data
Overview of functional TF binding sites in this region

00

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [0 / 0] on scaffold

This sequence was found using the sequence upstream the coding sequence of the exon2 of Pitx with a blast on the JGI site.

Show sequence

Length: 743

CAGCCATTTACAGTTTTTTTTCAGCTTTTTTGTATGTTGGGGAAATAACAAGTTAAAAAA
ATGAATAACACATTTAGAACGTTCATTCGCAGTATTTAGCAGTTTAAATGTTTTTATTCC
ATTTATTTAACAATTCTGTGTTTTTTTTAGAAGAATCAGTCGAAAAAATGGAGGAACTGA
ATCCAGCAAGCGAAGCAGGCGGCAGTGAAGGCGAAACCGACAAGAAGAATAACAAGAACA
CAAATAACAACACAGACACAGAGAAATCTAGTCCAGGGAACGCATGTAGTGACGATGCAG
ATGGCGACAAAAGCAAAAGACGACAACGCAGACAGAGGACACATTTTACTTCACAACAGC
TACAAGAATTGGAAGCATTATTCGCACGAAACAGGTACTATGACGTAACAAAGTAATGTT
TTATTTTAAAATTTAAAATAGTTGAAATCATTTTAACACGTGTTAGGTTGGTTTGCGTTT
TTTATGATTTGTTTCGATTTTTGTATTTAATATTTAGTATACGTGAACAGATACATTATG
AATAAGAAATAGTCGTTCGGTTCGGTTGCAGACTTTGTAAAAATAAATATGTTATTTTTT
ATAGGTATCCTGATATGTCAACGAGAGAAGAGATATCAGCTTGGACAAACCTTACCGAAG
CACGAGTCCGGGTAAGAATAAAACATAATATGACTTAAAACTACGCATTTTGACAGTAAA
GAGAGTAAAAGAGCTGGTATAAA

Reference(s)

Christiaen et al. (2005). A modular cis-regulatory system controls isoform-specific pitx expression in ascidian stomodaeum. Dev. Biol. 277(2):557-66

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