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CIS-REGULATION

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  1. Region 'REG00000873'

Cis-regulatory Region

Name

Ci-Pitx -2751/-2540

Short name

n/a

Region ID

REG00000873

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Maximilian Haeussler (2011-08-09)

Annotator

Clara Degos (2011-08-09)

Curator

Delphine Dauga (2011-08-09)

View position in WashU

Description

Minimal enhancer upstream of Pitx gene from -2751 to -2540, called D1abcde in the paper. This region can drive expression in the neurohypophysis primordium.

KH transcript model was taken as reference for the region s coordinates.

Hierarchy of Regulatory Regions

Modification of Ci-Pitx -5500/-1328 by deletion

Regulated gene(s)

KH2012:KH.L153.79 (PITX1; PITX2; PITX3)

Construct(s) made to test this region

CONS00000835: pCi-Pitx -2751/-2540:CES2:LacZ

Show in situ data
Overview of functional TF binding sites in this region

00

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Paired n/a CGCGACGA [-2,748 / -2,741]
2 Homeobox n/a GATTA [-2,732 / -2,728]
3 Fox n/a TAATATTTCTTT [-2,724 / -2,713]
4 MAD n/a GCCG [-2,705 / -2,702]
5 Homeobox n/a TGACAA [-2,688 / -2,683]
6 Homeobox n/a TGACAG [-2,677 / -2,672]
7 Fox n/a AAAAAACACTA [-2,640 / -2,630]
8 Homeobox n/a TAATC [-2,628 / -2,624]
9 MAD n/a GCCG [-2,583 / -2,580]
10 MAD n/a CGAC [-2,572 / -2,569]
11 MAD n/a GCCG [-2,550 / -2,547]

Sequence

Aniseed Coordinates: [0 / 0] on scaffold

The sequence is based on the first codon on the KH transcript.

Show sequence

Length: 211


AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGTCGGT
TCCTGACAATACACTGACAGGTAGTGCGAATAAAAGGCGCTCGTGGATGTAAAAAAACAC
TAATAATCTTCAGTAATTTCCGTGCCTTTCCTATGGGCGAAAACTCCCGCCGTGTGAAAC
GACTTCAAAAGGCGGACAGTCGCCGCCAAGG

Reference(s)

Haeussler et al. (2010). A cis-regulatory signature for chordate anterior neuroectodermal genes. PLoS Genet. 6(4):e1000912

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