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CIS-REGULATION

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  1. Region 'REG00000870'

Cis-regulatory Region

Name

Ci-GAD -1198/508

Short name

n/a

Region ID

REG00000870

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Katsumi Takamura (2010-07-21)

Annotator

Clara Degos (2019-02-25)

Curator

Delphine Dauga (2019-02-25)

View position in WashU

Description

Region upstream of Ci-GAD from -1198bp to 508bp.
This region (dCi-GAD up in the paper) can drive expression in the nervous system, more precisely in the GABAergic neurons.

Hierarchy of Regulatory Regions

Regulated gene(s)
n/a
Construct(s) made to test this region

CONS00001517: p.Ci-GAD -1198/508>unc76::eGFP

CONS00000834: pCi-GAD -1198/508::LacZ

Show in situ data
Overview of functional TF binding sites in this region

6,8268,531

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [6,826 / 8,531] on scaffold KhS761

Sequence found thanks to the primers used in the paper. Coordinates are based on the first exon of the KH transcript (KH.S761.6.v1.A.ND1-1).

Show sequence

Length: 1,706

TAATAAGCCCGACACTCGAACTTCGGAACCTCCAAGCCGATTGTTTCTGCATCTTCGCGA
ATAATATGCATTGTTACGTATATCATCTTATATAGAGGAGGCAGAAAAAAGGCAATTTTC
CGCTAAGACAAGCTTTATTACGCATGAATTAGTCGCATTGTGGGTTAATTGCGAATCCAT
GTCCGAATAATAAATACCGCCTCTCATGGGAACATATTCATTTGGATTTTGCCGCGTGCC
AATAAATTATTCATTTAAATGACAAAAAATACAATTCTAAAATCAACAATAGCTCTTTAC
CAGTAGTTCTGTTACGTCAAAGTACCCATTAGCTAATAAATAATCTTCCATTATTATCGA
TTGTTTTAAAGACAATCGCTAACTAGAATCATCAATCACGTTTATGACGTAACAATCAAT
TGTCAGTGCGAAATATGGTAGCGGTTTCAACATAGATATCTTAATAATAAACACATGTTT
AAACATCTATGTTGCCATGACATTTACGTCATATTTTAGAATCCATTTTGTTGTTACGTG
ACGTCATATTAACATTCTGGAATACGACGTTCATGGGAAATACCCTGATTCGATACAATG
GATTTGAAGGTAAAAAAAGTTGAGTATTTCACAAAGATTTTAAACTAACTCAATATTTTC
CAGGATTTGTTAAAATGCGAAATCTGCGGCGAATGTTTCAAGGATCCCGTAATGTTGGTG
CCATGTTCGCATAATTGTGAGTATTTATGTTTAATCTTTAAACGCACATTTCAATATCTT
TGTTTTAGTTTGTTCATCATGCATTGAACGCAACGATCGACAATCGTGTGTGATTTGTAA
GAAGGTAAATATATAAAACAAATGAAATAACAATAAAAATAAGATTTGTTGCAGAAATAT
TCTTCATCTCAAATTGTACCAAACATAATTATTCAACAATTTATCAAATGTTTACAAAAT
AAAAGGTAAAACTTCAAATAATCATCCTATCTTAAAATAAATCTTTGCATTTTAAACTTA
TGCTGTTCCAGACCAACGCACCAAATCAACCCCAGCCAAGTTAATTTAGAGAAAAAATAC
AAAGAAATAATTTCTGAAGCACAAACTTCAGTTCATAGTATTGACTTGTTTATGGGGCAT
TGTGATGTCATACAACCTGTTGTGACATGTAAATATAGTCCGGGGAGAAAAAGTATGTAA
CAATTAAGTTAACAATGAGCATTGTTTGATATTAATTCACGACAGAAGTTTTATGTCCCT
CGTGTGAAAGTTTTGTCGCAAAGAATCAAATTAATTTTCACCTCGATTCGTGTCTTGCAA
GAGAAGAAAAAAGAGAATGTTTAAGGAAAAAACAAAGAAAGTAAATTAGGAATACTTGGT
TAAAAGTTTTGTTTAATGTTTTTTATATTTAGTAAAATTTCCACCAAGCATAATTCATCA
CGTGACCTGGTTTCGACCAATGAGATTGCTGACGAACACTCAATGCCAAAGGAAAAAGGT
TAAACTTTATAAATTATCATTAAATTGAAATTAAACTCTCAATTACAGCAGATAAAACAA
ACTTGGACCTAATGCTTAAAGACGACAGCCAAGCAGATGAAATAAAACAATTTATTGATG
CAACATTTTCTTAATAAACGATTTGAATGATTTTACCGTGTTCGCCAAACATTATCCAGT
GTTCGAAGCAACTGAATTCCAGCGAG

Reference(s)

Stolfi et al. (2015). Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature 527(7578):371-4

Takamura et al. (2010). Neural map of the larval central nervous system in the ascidian Ciona intestinalis. Zool. Sci. 27(2):191-203

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