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CIS-REGULATION

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  1. Transcript 'Moocci.CG.ELv1_2.S61...'
  2. Clone 'cijm006c18'
  3. Region 'REG00000684'
  4. Region 'REG00001383'
  5. Region 'REG00000861'

Cis-regulatory Region

Name

Ci-Tbx6b -145/-13 E-box mutated

Short name

n/a

Region ID

REG00000861

Status

Not Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Jamie E. Kugler (2010-07-09)

Annotator

Clara Degos (2010-07-09)

Curator

Clara Degos (2010-07-09)


Description

Region upstream of Ci-Tbx6b from -145bp to -13bp. This region can drive muscle expression from neurula stage.
Region named 112bp Ci-Tbx6b CRM fragment in the paper.
Binding sites are present in this region : one E-box site, one CREB binding site and one T-box site. Here the E-box site was mutated (CAACTG=>ACCAGT). This mutation has no effect on expression.


ANISEED coordinates are different from the coordinates in the paper. Coordinates are based on KH transcript model.

Regulated gene(s)
n/a
Overview of functional TF binding sites in this region

26,96027,091

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 T-Box n/a TCAGTGTGAC [-25 / -16]
2 bZIP n/a TGACG [-19 / -15]

Sequence

Aniseed Coordinates: [26,960 / 27,091] on scaffold KhS654

The E-box site was replaced by the mutated sequence (CAACTG=>ACCAGT).

Show sequence

Length: 132


GAGACTCGTTTTCGATGCCACTTTGATCTTAACGAGTCATTAACAAGGAACCAGTCTTCC
TGATAAGCCTCCGAACTCTCCTGCTGATTCAAGTCTAGCGCATGCAGAACACTCCAAAAC
TCAGTGTGACGC