- Transcript 'Moocci.CG.ELv1_2.S61...' >
- Clone 'cijm006c18' >
- Region 'REG00000684' >
- Region 'REG00001383' >
- Region 'REG00000861'
Cis-regulatory Region
Name
Ci-Tbx6b -145/-13 E-box mutated
Short name
n/a
Region ID
REG00000861
Status
Not Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(extended_promoter)
Author
Jamie E. Kugler (2010-07-09)
Annotator
Clara Degos (2010-07-09)
Curator
Clara Degos (2010-07-09)
Region upstream of Ci-Tbx6b from -145bp to -13bp. This region can drive muscle expression from neurula stage.
Region named 112bp Ci-Tbx6b CRM fragment in the paper.
Binding sites are present in this region : one E-box site, one CREB binding site and one T-box site. Here the E-box site was mutated (CAACTG=>ACCAGT). This mutation has no effect on expression.
ANISEED coordinates are different from the coordinates in the paper. Coordinates are based on KH transcript model.
Modification of Ci-Tbx6b -145/-13 by substitution
- Ci-Tbx6b -438/-295 REG00000640 [ regulatory_region (enhancer) ]
- Ci-Tbx6b -2339/-13 REG00000853 [ regulatory_region (extended_promoter) ]
26,96027,091
Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
---|---|---|---|---|---|
1 | T-Box | n/a | TCAGTGTGAC | [-25 / -16] | |
2 | bZIP | n/a | TGACG | [-19 / -15] |
Aniseed Coordinates: [26,960 / 27,091] on scaffold KhS654
The E-box site was replaced by the mutated sequence (CAACTG=>ACCAGT).
GAGACTCGTTTTCGATGCCACTTTGATCTTAACGAGTCATTAACAAGGAACCAGTCTTCC
TGATAAGCCTCCGAACTCTCCTGCTGATTCAAGTCTAGCGCATGCAGAACACTCCAAAAC
TCAGTGTGACGC