- Transcript 'Boleac.CG.SB_v3.S148...' >
- Region 'REG00000860'
Cis-regulatory Region
Name
Ci-Tbx6b -145/-13 T-box mutated
Short name
n/a
Region ID
REG00000860
Status
Not Curated
Origin
engineered region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(extended_promoter)
Author
Jamie E. Kugler (2010-07-09)
Annotator
Clara Degos (2010-07-09)
Curator
Clara Degos (2010-07-09)
Region upstream of Ci-Tbx6b from -145bp to -13bp.
Region named 112bp Ci-Tbx6b CRM fragment in the paper.
Binding sites are present in this region : one E-box site, one CREB binding site and one T-box site.
The T-box site was mutated (TCAGTGTGAC=>TCAGTTGTAC), this mutation results in a loss of the muscle expression.
ANISEED coordinates are different from the coordinates in the paper. Coordinates are based on KH transcript model.
Modification of Ci-Tbx6b -145/-13 by substitution
- Ci-Tbx6b -438/-295 REG00000640 [ regulatory_region (enhancer) ]
- Ci-Tbx6b -2339/-13 REG00000853 [ regulatory_region (extended_promoter) ]
26,96027,091
Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment | |
---|---|---|---|---|---|
1 | bHLH | n/a | CAACTG | [-96 / -91] | |
2 | bZIP | n/a | TGACG | [-19 / -15] |
Aniseed Coordinates: [26,960 / 27,091] on scaffold KhS654
The T-box site was replaced by the mutated sequence.
GAGACTCGTTTTCGATGCCACTTTGATCTTAACGAGTCATTAACAAGGACAACTGCTTCC
TGATAAGCCTCCGAACTCTCCTGCTGATTCAAGTCTAGCGCATGCAGAACACTCCAAAAC
TCAGTTGTACGC