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CIS-REGULATION

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  1. Transcript 'Boleac.CG.SB_v3.S148...'
  2. Region 'REG00000860'

Cis-regulatory Region

Name

Ci-Tbx6b -145/-13 T-box mutated

Short name

n/a

Region ID

REG00000860

Status

Not Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Jamie E. Kugler (2010-07-09)

Annotator

Clara Degos (2010-07-09)

Curator

Clara Degos (2010-07-09)


Description

Region upstream of Ci-Tbx6b from -145bp to -13bp.
Region named 112bp Ci-Tbx6b CRM fragment in the paper.
Binding sites are present in this region : one E-box site, one CREB binding site and one T-box site.
The T-box site was mutated (TCAGTGTGAC=>TCAGTTGTAC), this mutation results in a loss of the muscle expression.

ANISEED coordinates are different from the coordinates in the paper. Coordinates are based on KH transcript model.

Overview of functional TF binding sites in this region

26,96027,091

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bHLH n/a CAACTG [-96 / -91]
2 bZIP n/a TGACG [-19 / -15]

Sequence

Aniseed Coordinates: [26,960 / 27,091] on scaffold KhS654

The T-box site was replaced by the mutated sequence.

Show sequence

Length: 132


GAGACTCGTTTTCGATGCCACTTTGATCTTAACGAGTCATTAACAAGGACAACTGCTTCC
TGATAAGCCTCCGAACTCTCCTGCTGATTCAAGTCTAGCGCATGCAGAACACTCCAAAAC
TCAGTTGTACGC