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CIS-REGULATION

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  1. Region 'REG00000826'

Cis-regulatory Region

Name

Ci-fibrn -2515/38

Short name

n/a

Region ID

REG00000826

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-05)

Annotator

Clara Degos (2010-07-06)

Curator

Delphine Dauga (2010-07-15)

View position in WashU

Description

Region upstream of Ci-fibrn from -2515bp to +38bp, driving notochord expression.

The corresponding construct is named p(-2.5 k)fibrn/lacZ in the paper.
Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Hierarchy of Regulatory Regions

Modification of Ci-fibrn -3016/38 by deletion

Regulated gene(s)

KH2012:KH.C1.832 (FGL1; FGL2; FIBCD1)

Construct(s) made to test this region

CONS00000789: pCi-fibrn -2515/38::LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,753,0561,755,608

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,753,056 / 1,755,608] on scaffold KhC1

Sequence found thanks to the primers used in the paper.

Show sequence

Length: 2,553

TGGACAGCATTATAACCTGCCTTATAGTGTATTGACCCCTGCATGGAAAAAGATGTATAT
GAAAATAGACAAACTGGTACATTTAACGGTAAGTCAACGAAGTGTTAAACTGGTACATTT
AACGGTAAGTCAACGAAGCCGTATAAATAGTCAAACTATAACTAAAAGTGCTCATTCGCA
TACTGCAATAGTTTCAGCAATACGACTGTGGGCATAGCAACTACTGATGTTATAATCTGT
ACTAACCGCTATACCTGCGTTTGCTTTATTAACTACGTAATATACATACTACCTTGTAAG
TTGGTACGAGGTGTATAAACACCCGCGTTATACCGACTGTCATGTTCCGGCCACGCGAGG
ATAAAAAAGTAAGTTACAAGTTATAAGTTATTCCACATTGTACAGTACATGAAGCGTTTA
TATAGAGCGGACTTTACCATCCGTTCAATCCACATTACTGGCTATAAGTTGAGCTTAGCA
TCTTAATACTGATGGCCGCAGGATGTATTGCTGTCTGTACATACAAACCTAATCTTCACC
CGATCTCTGCACATTGTTTACATTAAGTTAAGCCATCTATACAGTTTAAGTTAAAAACTC
AATGTGTTTTTGAAATATTTTAGAATAGCTGACTAGAAATAAATCTGCAATAAAACATAT
CGTTCAAAATATATACCTTGTTTAAAAGCAGCATTTATAAAGGTTTACACTGGTGCTTTT
TTTGCAACACGCCAATTTTTTAAATATATAGGTATAAATTACTTTTTTTTATTCCAAACA
GGCCAGTTCCTAACATTTATAACTACCCAGGCCCTCAATAGTCCAAGCATTCATATGCTC
TACTAACGGGTTGTCGCCAGCTTATATATAGGCCATTTAAAATGTGTCTGTTCAGATCAA
AGTTAGGGCCCAACGAGCCCAAAGCTAAATGCCTAAAGCTCAATATACGTAATTGTTATA
TATAATGCATTGTTTTCTGCCCGCTACACGAAGTGACGTAAAACGCTACAAGCTTAATTT
AAACGGAATCAGGTAATCTACATCGGCCTGTGACGCCAATTTCAAACACTGTGGCAAATT
TTTAACCGCCGACACTTTTTCAAATCCGATTTATTTTAAAGAAAGTCGTGCTCAATGTCG
CTTTAAAGTAAACACCATGTATGTATGCTGTATTCATGCACCTCATGCTTTCATACACTG
TCGAGTTATTACATGGTATCTTCTTATACACCGGAGTCTTATGCACTACAATACGAATAT
GTTATACTCACTCCATTACACCAAAATACTCAATAAAACTTTTATATTCCTGCTATACCC
GGCTTACAGTTATTACTATTAAACATTCCCACGCTGTATAAACTTTCTATTAATAACTTT
CCTAATATTCCCCGACCCAGCACTATAGGGAATTGTGATTAACACGACGCCACCGTTTTA
CCACTGTGGCTAAAATAGGAAACCGTAGTTTTGTTGTCATGTTTACGCAAAGTTTGAGCC
GCAAACACGTACATCGACTCTGTGATGTAAGATCGTAATATATTCTTATCAACAATTATG
TAGGAGGGTGTTGTGTCCGAATGTAAATTTTTAGACCTACTTAATTAATATAACATAATG
TTGTATTTAAACGCATAAGTACCATGCTATATTTGGATCATATATCCGAACCTGTGCTCA
TTTTTGAATTTTATACAATAAGTGTTATATATAATATAAAAATACGCATGTGTCAAACAC
GCAATCTCTTGTGCCTTTCCAAATGTCCTGCGCGCAATTTTGCTAAGTTTGACAGTTTTA
CCGCGTGTTTGATTATGCTGCTCTATTCTCCTTATGGCATAGTGAATGCCCGCACTCGAA
ACTTCTAAAGTGAAGCAGAAGAAACTGAAGCCAGGGTTTTAAACCGCGCTGTCCCAACAT
TGAGGTGTCGTGTTATTAGCATAGGGTTGATCAAACTCTAGCCTTGGTGTTACTTACCGC
ATGTCCCAAGTTCATTACGTCAGCGACGTTTGATGTTGACGCTTTCTTTATTGTTTTGAC
TGAGATCTGGCACGCGAGACATCGCATCTACCTGCGACATTAGATTGGCGGTTTTTTGAA
CGTTGTTTACGAATCTTCTTTACAGCGCCGTTGCTCAGTGGTTAGATCGTTTGCAATTTA
GTGGTCACTATAAATGGGTTGTCCAAATTATTAGCCACACATAAAAAAAATTAAACAATA
ATCACCTACAAACTAACGTATACTTAGTTACTCGTAATAGCTGGCAAGAGGTGTATGAAC
GCCCATATTATAACGACTGTCGTTTTCTGGCCACGCAAAGTTAAAGTAAGTTGCACTCTG
CCATAACTGGCGGTCTAAAGTTGTCCAAAAAAGCATTCCCAGCAATTTGCATATGTGGTA
AAGTAGCCGGGCAATGACGTAATAACACGGGTGTTCTGTTTCATACTCGTCGCCGTTTCC
GAGTTACCACGTAGGCTATGTGACTTTTTAGATGATTACTTCTGCCTTTAACGGTTTGCT
ATACAATTTATTGCCTTATTCGTGATTGTTTTA

Reference(s)

Takahashi et al. (2010). Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. Zool. Sci. 27(2):110-8

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