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CIS-REGULATION

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  1. Region 'REG00000822'

Cis-regulatory Region

Name

Ci-ERM -3052/76

Short name

n/a

Region ID

REG00000822

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-05)

Annotator

Clara Degos (2010-07-19)

Curator

Delphine Dauga (2010-07-26)


Description

Region upstream of Ci-ERM from -3052bp to +76bp, driving notochord expression.

The corresponding construct is named p(-3.0 k)ERM/lacZ in the paper.
Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Construct(s) made to test this region

CONS00000785: pCi-ERM -3052/76::LacZ

Overview of functional TF binding sites in this region

4,853,4894,856,616

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,853,489 / 4,856,616] on scaffold KhC12

Sequence found thanks to the primers used in the paper : Ci-ERM (BamH-R) CGGGATCCATTATTACATCACAGTAAGGAC and Ci-ERM (-3.0 kXba-F) GCTCTAGACTGGTTAACGTGTCTCGCCA.

Show sequence

Length: 3,128

TTCCAAACATTATTACATCACAGTAAGGACAATATTATCTATGATGTCACAATCACAAAC
AATTACCTGTCGAGTGCTACGCAAACCAACTTAAACACATCCCCTTATAAGGCAATACTT
GTGTATAAGTACGCAGAACATTGTTGCCTGCAACCTTATATGCTAATCTTTATCTACTTC
AACTTAACTTTTAAAACCAATCTCCTTTTACATTTTCAATGAAAACAAACTTAGAGAAGT
CTAAGCTTATAGCAAATTATTTAAAAACCCATTTTGTATCAAGAAAAGTACCTGAAACTC
ATTTTATATCGAGAAAAATATCTAAAACTTTTAAAATATTTTTTTTTTAACCAATTTTTT
TAAAAGGGGGAAAAAATTAATCTAAACCGCATTTAGCAGGTAACGCAATTTGACGCTACT
AGCCTACCCTACAGCACATCTAACTGGGTGTATAACACTGCATTCCTAGAGCACCAATTG
CTCTGCTATACATCAGTGTGGATACAACCTTTTATATTCCCAGAAACCCCAGTACAACTA
TATAGGTTGAACCATGTTTAGCGGGAAATCTAAGGCAACAGTGGTGGTCTATATTAAGGA
GCTTGGACTTTTAAACAGAAGTGACAACGGGGAAACCCAAATAATCTCATTCCAGATACA
TTACCCACAAGTTAGGAGTTAAAGCTGTTACCCGATGACAAGCATTGAACATAGATAGCT
AACAGAGCTTAGCTACACTTGTATGGATCTATGCGCCAAGCGTGTATGAGGAGCATTTGC
TTGATTATCTAAAGCAATAGTATTGAATGCAGGAATCATATTTAATGGGAGTTTACTGTT
ATATTGCTAATTAAAAACCCCAGTTTAATACTTCGGTGACTTTTATTTACTATGGGTTAC
TTCATGGTTACATATGTGGTAAATTGTAAGTGGGAATGAGGTGTATAAAACCAGAACACC
AATGGTATAATTACTATTATTGCTCACCATTCAAAGATAAATAAGTTACATTCATGAATT
TATATTTTTTTGAGATGGGAAAAAGTTGCATTGATATAGGTAAAGTTAACCACTGTGTTT
TAAGTCTTATATAAAAGGTGTGAATTAATCTAATAAGAAGTCACATTACATATTATAAAG
TATAGGAAATTTAGTAATTTAGATGCCTACAGCCTACAGCCTACAGCCTACAGTTATAGT
GATGAAAGATTACAGAGCTAAAATATTTCCGAATTCCCCAAGCACCCACGCATGCCTGTA
GACGATTACCGGTATTATAAATATTCAAAACTCGCGTAAATTGCAAACGAAACCAACCCG
TGTTTAAACCGCATTCACATCCGTAGCATAGACCTGGTTACTTTCAACTTTTCATCGCTG
TTATCTGGATTCCTTGCGTGTTAGTTTGTGGTGAAATATGCGGTTATCTGTTAAGGCGTT
TCTCATATAACTACCAGCGTAAAACTTTAATTTGGCATCAGTAGCCACGCTTATAAATCG
CTTTTGTTTTTCGGAGATAAAATTTACGTTATGGCATTGTTATGTATTATATTTTGGTTG
CGCGTAAGAATCAATTGATTTGAAAAGGGTTGTGCTGTATATGTAAAGCTTTGTATTTGA
TAGTTATAATACGGGTATGCGGTGCGTGTATTGTAGTAGTTTGGTGTTACTAATGTAATA
ATACGCAGATGCTAAAGCTATGTTAGTTTCAGCGTGGGTAAAGTAGTTCTTAAATAACAA
AACACACGTTTAACGTTACGGATAACGGCCCAACTAAACGTATGTAAGTCGGATGTATTA
TGGGTATTAGACTTAATTCTATATCGTGTATTAGCGACCATGTAATACGCCTCCGTAACT
AGTGCTTGTACATCAAACATGTGTAATTAACTTTCGTACACCACGCGTATAGATAAGTAG
GGGTGACGTCATTTTGTTGGCGCAACGCATGAAAGCGGGTTTTAATATAAAATACATTTT
TTTATCCCTTCGAAAGCGGTTACGAAAATCGTATTATTAAACGAGGTAGAGAACGAAATC
AACAGCAAAACACGCTATGGGTAAAAGGTATAGAAAGAGTCAGTTAAAAATCGTGGCTGT
TGAACCCAAAATCTGCAGACCATGAAAAACTGCATTCGTGCGTTGACGTTTTAACGGTGT
GCGAGCATACGACACGTAAGCATACTGCAATGCTGCAAGAAATTCTTACAAATTATGACG
ACCACTCGTCTACGTCACAGGGAGAAATAGTTTTTAGTTTTAAGCAAACTATACGTTGAA
GAAACCTTCGTTAGGTTACAAATTATTTTAGTTTATACGTTAGGGCTATAATTCTTCACT
AGCACCAACGGTACACTATACTTTAAAAACAACATTTTCTGGAATTTTAGTAAAACATAT
TTGTCTTAGCGGGAAGCATCTCTAAACAACCTAACTATAACCTTACGAAAATACACATTT
ACAACGTAACCGTTATATTACGTTCACATTGAACTTAAACCAACTAATGCTACAATGCAT
TGGCTTCTACCAAGCCTCATGATGCGATGCTCATACATTATTCACCCCTGCTGTGCGTTC
TATACAAGCAGCACGACGTCCGTTATCGATTCGAGAATTCCCAACTGTGACGTCATTAAT
CTCGCGACGTAGTGTGACGTAACAATCAAATAATCGACGTCGATGTGATGACGTGTTAAT
TACGAAACAATGTTGGTTTGGCAAATAAGGAAATTAGTTTAGTAGACTTTGATGTAAAGA
CAAGTTGAAAAATAAGTTAATTACGAGGTATTTTATCAAGGTAATTAATCATAAGAAATG
TTACATGAAGACTGTATTTTACGGTAGGTGTTACCGTGTTACATACCAATACAGAATGTG
GAAAAAAATGTCCAATTACGGAGTATTGCTGAACTACGACTTTGTATTTTATGTTTCTTG
TACTTCATGAACATAACGTGAACTTTTATTTACCTCGTATTGGTAAAACGTTAACATCGA
ACATTAAATCCTTTCGTTAGAAAAAGTATTTGTATTTTAACATAAGTTAAAGTTATGTAT
AAGAATATTTCCAAAGGGAAGTAATGTATTTTTAAACGTGTGGCGAGACACGTTAACCAG
GGCACGTA