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CIS-REGULATION

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  1. Region 'REG00000814'

Cis-regulatory Region

Name

Ci-Noto1 -407/41

Short name

n/a

Region ID

REG00000814

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-02)

Annotator

Clara Degos (2010-07-19)

Curator

Delphine Dauga (2010-07-26)


Description

Region upstream of Ci-Noto1 from -407bp to +41bp. This region is required for Notochord expression.

The corresponding construct is named "p(-0.5k)Noto1/lacZ" in the paper.

Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Regulated gene(s)

KH2012:KH.L20.18 (KH.L20.18)

Construct(s) made to test this region

CONS00000777: pCi-Noto1 -407/41::LacZ

Overview of functional TF binding sites in this region

513,733514,180

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [513,733 / 514,180] on scaffold KhL20

Sequence found thanks to the primers used in the paper : Ci-Noto1 (BamH-R)
CGGGATCCATAACTTTGGATTATTTGT and Ci-Noto1 (-0.5 kHind-F)
CCCAAGCTTGACCAGTTGGCAGAAAG. Coordinates are based on the first exon of the KH transcript.

Show sequence

Length: 448

AAGCTCTAAGGCAGACGTTTGCATATATGTGCATAAGGTGTATGGTACTGTGGGCTAAGA
TACCGTTAGCAGATGATAATAAATATTCACTATTTTCTAATCGGGTTTTTAACAATTAAC
AACGCTCTTTAAGACTCGAGATGATACGAATATATATTTCTGTAAAGATTTTCTTGTTTA
CTATTAAATGGGTCGAAAAAAGAGAACGAAAAACTGACCCATCTTACCCCAACCTGATAG
ATATTTCTTTGTTTGTTCTTTTTACATCCAGGCAAGTAACGCTGTAGTGTAAAAGTCGTT
TAAAAAAAATAAACAAAGAAATATCTACGCTAAAAACGCTGGGTGTATATAGATATGATG
GGCAACAAAACCCAATCAAATACGTTCATTTAAAATGTTCTTAGTAGACAGGTAAACTAT
TACAAATAATCCAAAATTATGAAGTTGT