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CIS-REGULATION

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  1. Region 'REG00000811'

Cis-regulatory Region

Name

Ci-Noto1 -2078/41

Short name

n/a

Region ID

REG00000811

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-02)

Annotator

Clara Degos (2010-07-22)

Curator

Delphine Dauga (2010-07-26)


Description

Region upstream of Ci-Noto1 from -2078bp to +41bp. This region is required for notochord expression.


The corresponding construct is named "p(-2.0k)Noto1/lacZ" in the paper.

Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Regulated gene(s)

KH2012:KH.L20.18 (KH.L20.18)

Construct(s) made to test this region

CONS00000774: pCi-Noto1 -2078/41::LacZ

Overview of functional TF binding sites in this region

512,062514,180

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [512,062 / 514,180] on scaffold KhL20

Sequence found thanks to the primers : Ci-Noto1 (BamH-R) CGGGATCCATAACTTTGGATTATTTGT
and Ci-Noto1 (-2.0 kHind-F)
CCCAAGCTCCACGGTCGAGTTGCTGAA. Coordinates are based on the first exon of the KH transcript.

Show sequence

Length: 2,119

GCTATTGCAGTGTGTGGATAAGCAGACATGACTTTTGGTTGGTTTGATCATTTATATCCT
CGTGGGTGGGAACTTAAGTGACTTATCCATGGTTGCCACTCCCATGCCCACAGTCGAGTT
GCTGAAACTATTGCAGTGTGTGGATAAGCAGACATGACTTTAGGGGGAACCTGAGCAACT
TTTAAGTCTCTTTCACAGCTACATTCAATAAACTCCACTAAAAAATCTCTCGCTGCATAA
AATACAAATACCCTTTAACGAATATACATATAATACCCTTCATACGCCACAAGAACATCT
ACTGTATACTGATAACATACCACGAATGCAAATTCATTCTGAAAACATTTCATCCAAAAT
GCTATACTTGCACCTGTAAACACATTCTACGCATATTATAACCTCTAATCTCAAGCTGGA
GCCGAACAGCTGATATACAGCAAACTCCCATAACTGGATAACGAAGATTAAAATTACTTG
AAAGTCTTCCTGTAGTATGGTTACAATGCAAATGCGGTTTCTTATCTTGTGCATTACAAG
ATTACTTCGATGTTAGGAAGGAAGTCGATGTTTTTTTGTTCTTTCACAGCGTTAAACGCC
GCTTAAAAAGAACAAACAAGGAAATATCTACGCTGACCTGCGCTGAACAACGCTGGGTGT
AGATATTACTTTGTTCGTTCTTTTTTACACCGAGGCGAGTTACGCTGTAGTGTAAACGTC
GCTTAAAAGAACAAACAAAGAAATATCTACGCTGACCTGCGCTGAACAACGCTGGGTGTA
GATATTACTTTGTTCGTTCTTTTTACACCGAGGCGAGTTACGCTGTAGTGTAAACGTCGC
TTAAAAGAACAAACAAAGAAATATCTACGCTGACCTGCGCTGAACAACGCTGGGTGTAGA
TATTACTTTGTTCGTTCTTTTTACACCGAGGCGAGTTACGCTGTAGTGTAAACGTCGCTT
AAATATTTTTTTGACGAGCTATGGGCTTTTCTGTGTGAACGCCAAAAACCACCGGATTGG
TTAATATTTAAATACATTTATGACAAAACAACAGCTTTGTTAAACAACAAGGCTTGCATA
TGAACAAACAGAGGCCATGTATAGTTCCCATGTTGTTTACTTTAACTTCACTGTGGTAAC
AATCACTGTTTATTATCAAATAATTGCATTTCATTACCTTTAAAAGGGTGCAATGCCGTC
ATAATGCATATTAATTGCAAATAATTATAACAAAGGGTCATAATTACGACGTCATGTAGT
CAATTATGACGAAATAAAACACAAAATGTTTTTTTTTTTTGCAAAGTTAGTTAAAAGAAC
ACGAAAAAACGTGACAAAAAGATGATTCGGCGACAGAACGCGATTTGATTAATGACAATG
TATCGCGCCACCCGACTGTAACGTCACAGATATCATTTAGAAGACACGACTTATTTATGA
CGTAACAGATACGCTGGGTGTGGTGAAACAATGGCTATTATTTGGTCATAAATATTTGAG
TGACCTACAGGCACTTAGGTGCCAAAAACAAGAGCAATTAGTTGAGGCATGAACGCGAAA
ACGCTTTCTGTTGCAAGTGACCAGTTGACGCAAAGAGAGACAGATAAACGGACAGGAACA
GACAGTGTATTAAGAACTTGATTTAACTGATGTGGAACTCCTTTAGTGAGAAAGCTCTAA
GGCAGACGTTTGCATATATGTGCATAAGGTGTATGGTACTGTGGGCTAAGATACCGTTAG
CAGATGATAATAAATATTCACTATTTTCTAATCGGGTTTTTAACAATTAACAACGCTCTT
TAAGACTCGAGATGATACGAATATATATTTCTGTAAAGATTTTCTTGTTTACTATTAAAT
GGGTCGAAAAAAGAGAACGAAAAACTGACCCATCTTACCCCAACCTGATAGATATTTCTT
TGTTTGTTCTTTTTACATCCAGGCAAGTAACGCTGTAGTGTAAAAGTCGTTTAAAAAAAA
TAAACAAAGAAATATCTACGCTAAAAACGCTGGGTGTATATAGATATGATGGGCAACAAA
ACCCAATCAAATACGTTCATTTAAAATGTTCTTAGTAGACAGGTAAACTATTACAAATAA
TCCAAAATTATGAAGTTGT