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CIS-REGULATION

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  1. Region 'REG00000810'

Cis-regulatory Region

Name

Ci-Noto1 -2364/41

Short name

n/a

Region ID

REG00000810

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Hiroki Takahashi (2010-07-02)

Annotator

Clara Degos (2010-07-19)

Curator

Delphine Dauga (2010-07-23)


Description

Region upstream of Ci-Noto1 from -2364bp to +41bp. This region is required for notochord expression.


The corresponding construct is named "p(-2.2k)Noto1/lacZ" in the paper.

Be careful, coordinates in the paper may be different from ANISEED coordinates. We took the KH transcript model as reference.

Regulated gene(s)

KH2012:KH.L20.18 (KH.L20.18)

Construct(s) made to test this region

CONS00000773: pCi-Noto1 -2364/41::LacZ

Overview of functional TF binding sites in this region

511,776514,180

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [511,776 / 514,180] on scaffold KhL20

Sequence found thanks to the primers used in the paper : Ci-Noto1 (BamH-R) CGGGATCCATAACTTTGGATTATTTGT and
Ci-Noto1 (-2.2 kHind-F) CCCAAGCTTCAGTGTAAGTAGCTGCTTTG.
Coordinates are based on the first exon of the KH transcript.

Show sequence

Length: 2,405

TTAACACCACAGTGTAAGTACGTTCTTTGTAAGTCCAAGTCCAAAAGGCCGTGTAAGTAA
TGCATTACTTATTGGGAATAAAGGCGAACTGCAGTTTGTGGTTGCTGTTAAAATACAAAG
CAAAACATTGCGGCAGATTTTAACTCTTACTCAGTTCCTGGTTAATTATATATGTACTAA
CTGGCGCGCTCGCCTCGTAATTACTGAGTTTATAACGTTATGTAAACGTGAACTTGAGTG
ACTTATCCATGGTTGCGACTCTTATGCCCACGGTCGAGTTGCTGAAGCTATTGCAGTGTG
TGGATAAGCAGACATGACTTTTGGTTGGTTTGATCATTTATATCCTCGTGGGTGGGAACT
TAAGTGACTTATCCATGGTTGCCACTCCCATGCCCACAGTCGAGTTGCTGAAACTATTGC
AGTGTGTGGATAAGCAGACATGACTTTAGGGGGAACCTGAGCAACTTTTAAGTCTCTTTC
ACAGCTACATTCAATAAACTCCACTAAAAAATCTCTCGCTGCATAAAATACAAATACCCT
TTAACGAATATACATATAATACCCTTCATACGCCACAAGAACATCTACTGTATACTGATA
ACATACCACGAATGCAAATTCATTCTGAAAACATTTCATCCAAAATGCTATACTTGCACC
TGTAAACACATTCTACGCATATTATAACCTCTAATCTCAAGCTGGAGCCGAACAGCTGAT
ATACAGCAAACTCCCATAACTGGATAACGAAGATTAAAATTACTTGAAAGTCTTCCTGTA
GTATGGTTACAATGCAAATGCGGTTTCTTATCTTGTGCATTACAAGATTACTTCGATGTT
AGGAAGGAAGTCGATGTTTTTTTGTTCTTTCACAGCGTTAAACGCCGCTTAAAAAGAACA
AACAAGGAAATATCTACGCTGACCTGCGCTGAACAACGCTGGGTGTAGATATTACTTTGT
TCGTTCTTTTTTACACCGAGGCGAGTTACGCTGTAGTGTAAACGTCGCTTAAAAGAACAA
ACAAAGAAATATCTACGCTGACCTGCGCTGAACAACGCTGGGTGTAGATATTACTTTGTT
CGTTCTTTTTACACCGAGGCGAGTTACGCTGTAGTGTAAACGTCGCTTAAAAGAACAAAC
AAAGAAATATCTACGCTGACCTGCGCTGAACAACGCTGGGTGTAGATATTACTTTGTTCG
TTCTTTTTACACCGAGGCGAGTTACGCTGTAGTGTAAACGTCGCTTAAATATTTTTTTGA
CGAGCTATGGGCTTTTCTGTGTGAACGCCAAAAACCACCGGATTGGTTAATATTTAAATA
CATTTATGACAAAACAACAGCTTTGTTAAACAACAAGGCTTGCATATGAACAAACAGAGG
CCATGTATAGTTCCCATGTTGTTTACTTTAACTTCACTGTGGTAACAATCACTGTTTATT
ATCAAATAATTGCATTTCATTACCTTTAAAAGGGTGCAATGCCGTCATAATGCATATTAA
TTGCAAATAATTATAACAAAGGGTCATAATTACGACGTCATGTAGTCAATTATGACGAAA
TAAAACACAAAATGTTTTTTTTTTTTGCAAAGTTAGTTAAAAGAACACGAAAAAACGTGA
CAAAAAGATGATTCGGCGACAGAACGCGATTTGATTAATGACAATGTATCGCGCCACCCG
ACTGTAACGTCACAGATATCATTTAGAAGACACGACTTATTTATGACGTAACAGATACGC
TGGGTGTGGTGAAACAATGGCTATTATTTGGTCATAAATATTTGAGTGACCTACAGGCAC
TTAGGTGCCAAAAACAAGAGCAATTAGTTGAGGCATGAACGCGAAAACGCTTTCTGTTGC
AAGTGACCAGTTGACGCAAAGAGAGACAGATAAACGGACAGGAACAGACAGTGTATTAAG
AACTTGATTTAACTGATGTGGAACTCCTTTAGTGAGAAAGCTCTAAGGCAGACGTTTGCA
TATATGTGCATAAGGTGTATGGTACTGTGGGCTAAGATACCGTTAGCAGATGATAATAAA
TATTCACTATTTTCTAATCGGGTTTTTAACAATTAACAACGCTCTTTAAGACTCGAGATG
ATACGAATATATATTTCTGTAAAGATTTTCTTGTTTACTATTAAATGGGTCGAAAAAAGA
GAACGAAAAACTGACCCATCTTACCCCAACCTGATAGATATTTCTTTGTTTGTTCTTTTT
ACATCCAGGCAAGTAACGCTGTAGTGTAAAAGTCGTTTAAAAAAAATAAACAAAGAAATA
TCTACGCTAAAAACGCTGGGTGTATATAGATATGATGGGCAACAAAACCCAATCAAATAC
GTTCATTTAAAATGTTCTTAGTAGACAGGTAAACTATTACAAATAATCCAAAATTATGAA
GTTGT