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CIS-REGULATION

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  1. Region 'REG00000788'

Cis-regulatory Region

Name

Ci-Nut1 -51/94

Short name

n/a

Region ID

REG00000788

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Kotaro Shimai (2010-06-29)

Annotator

Clara Degos (2011-08-11)

Curator

Delphine Dauga (2011-08-11)

View position in WashU

Description

Region upstream of Ci-Nut1 from -51bp to +94bp, named -47bp. This region can t drive expression in neural tube.

The putative first ZicL site, and the Fox site were absent in this region. There is only the last ZicL putative binding site.


Transcription start site is based on the start of the first exon of the KH transcript model.

Be carefull the coordinates in the paper may be different from ANISEED coordinates.

Hierarchy of Regulatory Regions

Modification of Ci-Nut1 -77/94 by deletion

Regulated gene(s)

KH2012:KH.C14.4 (ADRB2; P2RY12; RGR)

Construct(s) made to test this region

CONS00000751: pCi-Nut1 -51/94 pVenus::EGFP

Show in situ data
Overview of functional TF binding sites in this region

1,413,7951,413,939

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 ZnF_(C2H2) n/a GTCGCTTTG [-41 / -33]

Sequence

Aniseed Coordinates: [1,413,795 / 1,413,939] on scaffold KhC14

Transcription start site is based on the start of the first exon of the KH transcript model.

Show sequence

Length: 145


TTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCGTACCAGTTTTACAGT
TTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACACTATATCAACAACAT
GGAAATTGATTTTGGATTTGCAAGA

Reference(s)

Shimai et al. (2010). Upstream regulatory sequences required for specific gene expression in the ascidian neural tube. Zool. Sci. 27(2):76-83

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