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CIS-REGULATION

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  1. Region 'REG00000702'

Cis-regulatory Region

Name

Ci-Pitx -2751/-2696 repeated x2/75bp spacer

Short name

n/a

Region ID

REG00000702

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Maximilian Haeussler (2010-06-04)

Annotator

Clara Degos (2010-07-22)

Curator

Delphine Dauga (2011-08-10)


Description

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)-75-(ab) in the paper. The region named D1ab in the paper was repeat once, and between the two fragments there is a spacer region of 75bp. This region can t drive expression in the neurohypophysis primordium.

KH transcript model was taken as reference for the region s coordinates.

Hierarchy of Regulatory Regions

Modification of Ci-Pitx -2751/-2696 repeated x2 by insertion

Overview of functional TF binding sites in this region

197,501197,555

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Paired n/a CGCGACGA [-2,748 / -2,741]
2 Homeobox n/a GATTA [-2,732 / -2,728]
3 Fox n/a TAATATTTCTTT [-2,724 / -2,713]
4 MAD n/a GCCG [-2,705 / -2,702]
5 Paired n/a CGCGACGA [-2,618 / -2,611]
6 Homeobox n/a GATTA [-2,602 / -2,598]
7 Fox n/a TAATATTTCTTT [-2,594 / -2,583]
8 MAD n/a GCCG [-2,575 / -2,572]

Sequence

Aniseed Coordinates: [197,501 / 197,555] on scaffold KhL153

The spacer region added was represented by "N".
The sequence is based on the first codon on the KH transcript.

Show sequence

Length: 185


AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNAAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCG
CCCAG