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CIS-REGULATION

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  1. Region 'REG00000695'

Cis-regulatory Region

Name

Ci-Pitx -2751/-2696 repeated x5

Short name

n/a

Region ID

REG00000695

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(complex_region)

Author

Maximilian Haeussler (2010-06-02)

Annotator

Clara Degos (2010-07-22)

Curator

Delphine Dauga (2011-08-10)

View position in WashU

Description

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)x5 in the paper. The region named D1ab in the paper was repeat 5 times. This region can drive expression in the neurohypophysis primordium.

KH transcript model was taken as reference for the region s coordinates.

Hierarchy of Regulatory Regions

Modification of Ci-Pitx -2751/-2606 delta -2693/-2646 by deletion

Regulated gene(s)

KH2012:KH.L153.79 (PITX1; PITX2; PITX3)

Construct(s) made to test this region

CONS00000664: pCi-Pitx -2751/-2696 repeated x5:CES2:LacZ

Show in situ data
Overview of functional TF binding sites in this region

197,499197,555

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 Paired n/a CGCGACGA [-2,748 / -2,741]
2 Homeobox n/a GATTA [-2,732 / -2,728]
3 Fox n/a TAATATTTCTTT [-2,724 / -2,713]
4 MAD n/a GCCG [-2,705 / -2,702]
5 Paired n/a CGCGACGA [-2,693 / -2,686]
6 Homeobox n/a GATTA [-2,677 / -2,673]
7 Fox n/a TAATATTTCTTT [-2,669 / -2,658]
8 MAD n/a GCCG [-2,650 / -2,647]
9 Paired n/a CGCGACGA [-2,638 / -2,631]
10 Homeobox n/a GATTA [-2,622 / -2,618]
11 Fox n/a TAATATTTCTTT [-2,614 / -2,603]
12 MAD n/a GCCG [-2,595 / -2,592]
13 Paired n/a CGCGACGA [-2,583 / -2,576]
14 Homeobox n/a GATTA [-2,567 / -2,563]
15 Fox n/a TAATATTTCTTT [-2,559 / -2,548]
16 MAD n/a GCCG [-2,540 / -2,537]
17 Paired n/a CGCGACGA [-2,528 / -2,521]
18 Homeobox n/a GATTA [-2,512 / -2,508]
19 Fox n/a TAATATTTCTTT [-2,504 / -2,493]
20 MAD n/a GCCG [-2,485 / -2,482]

Sequence

Aniseed Coordinates: [197,499 / 197,555] on scaffold KhL153


The sequence is based on the first codon on the KH transcript.

Show sequence

Length: 275


AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACG
CGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACGCGACG
ACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACGCGACGACCTC
CACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAACGCGACGACCTCCACGG
ATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAG

Reference(s)

Haeussler et al. (2010). A cis-regulatory signature for chordate anterior neuroectodermal genes. PLoS Genet. 6(4):e1000912

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