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CIS-REGULATION

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  1. Region 'REG00000624'

Cis-regulatory Region

Name

Ci-troponin I -746/66 MyoD mutated

Short name

n/a

Region ID

REG00000624

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Christopher D. Brown (2009-12-13)

Annotator

Clara Degos (2010-07-23)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

Construct #1518 in the paper. In this construction the Tbx6 and CRE sites were absent and the MyoD site was mutated.

Hierarchy of Regulatory Regions

Modification of Ci-troponin I -757/66 CRE mutated by deletion

Regulated gene(s)

KH2012:KH.C11.673 (TNNI1; TNNI2; TNNI3)

Construct(s) made to test this region

CONS00000594: pCi-troponin I -746/66 MyoD mutated::LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,684,4501,685,254

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,684,450 / 1,685,254] on scaffold KhC11

Transcription start site is based on the start of the first exon of the KH transcript model. In this construction the MyoD site mutated was remplaced by "-"

Show sequence

Length: 812

ACTG------TTATCGCCTGAGCAGCAGTGTTCCCCGTGATTTTTTGCTGTGATTCAAAT
TTATTGATTACAAATATAAAAATTATATTATATTATACTTAATCAATGTAAAGTATTGAT
ATAAGCATATTTCGTTAATTTAACCGAAATTAGCTATAGTTTAAGCAGTTTCCAGAGGTT
TACATTAGTACGGCGGCAACACTCGTTCTATATTAAATCCAGACAACAAGAACTTCTTCT
TCGTTTAGTCCTTCGATATTCTATTTAAGGGAGCTTACAGCATCCATCCAACATCGGTAA
TCAAGAATCTGTTAACCTTGTTTAGTCACAGAGAAAGGTTCATGCATTTAAGAACCCCTT
AGTGAATTTAAAGAATTTTCTTGTAGAAAGGGCCGCATAGTGGCCATGAATACTATGGTT
AACTATTAGCTTAGTTGAGTTTATCATGCTCAATATTTTTATCATTTAGATTTTCTGAAG
GGTTTAAAATTAAAAGCCTATTAAGCATATTTATTTAAATATAAGTTTATCCTCTCAGGG
TTGTGATAGTGATAACTTATCAGGATTTATAACTATAGCGCAATACTGAATTTATCAAGC
TGATAAGACTACCAGTACCTGCGTAAAACCTTTAGGAAGTCTAAATAGATACTTACTCTT
CAGTAACTGAATGCAACCTGTATAAACGGAATATTGCAGAGTTATATTTTTATCGTTTAT
ATCCTTGTAGTTCACTAATGCATTTTCATCTTCAGCAGTATCAATTGGTTAAGTAGGTCT
TAGTTTGGCTTAGCAACACAGCAAAACAGCAC

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60

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