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CIS-REGULATION

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  1. Region 'REG00000619'

Cis-regulatory Region

Name

Ci-troponinT -512/74 CRE1/2 mutated

Short name

n/a

Region ID

REG00000619

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Christopher D. Brown (2009-12-12)

Annotator

Clara Degos (2009-12-12)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

Construct #1358 in the paper. In this construction the Tbx6 motif was absent and the second and third CRE motifs were mutated.

Hierarchy of Regulatory Regions

Modification of Ci-troponinT -512/74 by substitution

Regulated gene(s)

KH2012:KH.C4.57 (TNNT1; TNNT2; TNNT3)

Construct(s) made to test this region

CONS00000589: pCi-troponinT -512/74 CRE1/2 mutated::LacZ

Show in situ data
Overview of functional TF binding sites in this region

5,221,7855,222,334

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bZIP n/a CGTCAC [-511 / -506]

Sequence

Aniseed Coordinates: [5,221,785 / 5,222,334] on scaffold KhC4

Transcription start site is based on the start of the first exon of the KH transcript model. The second and third CRE sites mutated were remplaced by "-".

Show sequence

Length: 585


ACGTCACCTACACAATGA------AGGTGA------GTTTTGAAAAAAAGGTTGAGTCTT
ATTCTATAAAAGGTTGGTCGGCAGCAACATAATCATTATCGAAGTCGCTTCAGCTTCATT
CCATTCCTACTTCAAAGCATCAGCTTCTCAGGTCTTAAAGTTGTAGCTTCAATATGTTCA
TACAGAACCAAGCAACAATTATTCAATATAGAAAAAGCAGTTCAGAAAGTTTATCTTTGG
CATAAATGTGACGTCATAGTGACGTACTCGGTAATCTATTTCACCGGTGCAATTTGCCAA
TTAGATTAATTTGGTTAAACCAAGGTATTCGAGAAATATGTTTCGCACAGTCGAATATCT
GCTTGTGATGTCATAAGCCAGCAACGTAGACAGACGTATCTTCAGCTGTCGTTCTGTGAC
GTCATAGTGGTATCGGCGCATTGTGCCGCGGTCGGCACCCATGGTCCAGTGACCATATAA
GTTCATGTTGTTTTCTTTCCATCACCAGGTTATAACTTGATTAGACTTTCTTACCTGACT
TGACCGTTTGCTCAAAAATGTCGGACTCAGAAGAATACAGCAGCG

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60

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