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CIS-REGULATION

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  1. Region 'REG00000618'

Cis-regulatory Region

Name

Ci-troponinT -497/74 CRE3 mutated

Short name

n/a

Region ID

REG00000618

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Christopher D. Brown (2009-12-12)

Annotator

Clara Degos (2009-12-12)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

Construct #1382 in the paper. In this construction the Tbx6 and the first CRE motifs were absent. Moreover the last CRE motif was mutated.

Hierarchy of Regulatory Regions

Modification of Ci-troponinT -497/74 by substitution

Regulated gene(s)

KH2012:KH.C4.57 (TNNT1; TNNT2; TNNT3)

Construct(s) made to test this region

CONS00000588: pCi-troponinT -497/74 CRE3 mutated::LacZ

Show in situ data
Overview of functional TF binding sites in this region

5,221,7855,222,356

Motif Name Binding Factor(s) Sequence Position in Region Comment
1 bZIP n/a CGTCAA [-493 / -488]

Sequence

Aniseed Coordinates: [5,221,785 / 5,222,356] on scaffold KhC4

Transcription start site is based on the start of the first exon of the KH transcript model. The CRE site mutated was remplaced by "-".

Show sequence

Length: 571


ATGACGTCAAAGGTGA------GTTTTGAAAAAAAGGTTGAGTCTTATTCTATAAAAGGT
TGGTCGGCAGCAACATAATCATTATCGAAGTCGCTTCAGCTTCATTCCATTCCTACTTCA
AAGCATCAGCTTCTCAGGTCTTAAAGTTGTAGCTTCAATATGTTCATACAGAACCAAGCA
ACAATTATTCAATATAGAAAAAGCAGTTCAGAAAGTTTATCTTTGGCATAAATGTGACGT
CATAGTGACGTACTCGGTAATCTATTTCACCGGTGCAATTTGCCAATTAGATTAATTTGG
TTAAACCAAGGTATTCGAGAAATATGTTTCGCACAGTCGAATATCTGCTTGTGATGTCAT
AAGCCAGCAACGTAGACAGACGTATCTTCAGCTGTCGTTCTGTGACGTCATAGTGGTATC
GGCGCATTGTGCCGCGGTCGGCACCCATGGTCCAGTGACCATATAAGTTCATGTTGTTTT
CTTTCCATCACCAGGTTATAACTTGATTAGACTTTCTTACCTGACTTGACCGTTTGCTCA
AAAATGTCGGACTCAGAAGAATACAGCAGCG

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60

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