Aniseed - Cis-regulation

Region 'REG00000507'

Cis-regulatory Region

Name

Creatine kinase -2606bp/-114bp Tbx6 mutated -548

Short name

n/a

Region ID

REG00000507

Status

Not Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Christopher D. Brown (2009-12-10)

Annotator

Clara Degos (2010-07-23)

Curator

Clara Degos (2009-12-10)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model.

Construct #1584 in the paper. It the same construction that 1576 excepted by a mutation in the Tbx6 site in -548.

Hierarchy of Regulatory Regions

Modification of Creatine kinase -2606bp/-114bp by substitution

Creatine kinase -2606bp/-114bp REG00000474 [ regulatory_region (extended_promoter) ]
- Creatine kinase -2606bp/-114bp Tbx6 mutated -548 REG00000507 [ inactive_region ]
- Creatine kinase -592bp/-114bp REG00000508 [ regulatory_region (extended_promoter) ]
  - Creatine kinase -571bp/-114bp REG00000509 [ regulatory_region (extended_promoter) ]
    - Creatine kinase -548bp/-114bp REG00000511 [ inactive_region ]
      - Creatine kinase -539bp/-114bp REG00000512 [ inactive_region ]

Regulated gene(s)

KH2012:KH.L10.5 (CKB; CKM; CKMT2)

Construct(s) made to test this region

CONS00000479: pCreatine kinase -2606bp/-114bp Tbx6 mutated -791 ::LacZ

Show in situ data

Overview of functional TF binding sites in this region

6,3158,806

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment
1	T-Box	n/a	tgtgcatgt	[-570 / -562]

Sequence

Aniseed Coordinates: [6,315 / 8,806] on scaffold KhL10

Transcription start site is based on the start of the first exon of the KH transcript model.

Show sequence

Length: 2,492

CGCCTTTATCAGAGCATATTAACTGTCTAGTCCAAGGACGCTCACATCAATGAGGACACT
GATACAATGATAGCAGCATGAAGCCTCGAAACCGTATATCATGTGGACTATACAGATTGA
CAGGCGCTTTCACCTTGTGTCACGAGACCAACCATTTTTATACATTTCATTTTATATGTA
TATGCGGACCGTAACAGCATGTGCATACAAAGAACTTATTTATATTGCTATACATTAATA
TATTTTTACACCTTCGTAAACTGTGAACATTATACATGATTAAGATGCAGGATTTGATTT
TTTTACCTTGATGTTTGTCTTGATAGAGTTGTACGTGCAGTAGCATAACTAATAAACAGC
GAAGTAACAACGCAAATTAACATTATATTTTTCATATTGACTAAAAAGCGCTTTCGACAA
TCTAGTCTATGGCAGTATTTAAAATAATATAATGCAAATTTTTGTTAAATTCATTAGACT
AATACATACTATATATGTCATTTAAAAACAGGAATCGTAACTAATCTGCCAGAAACTTTT
TGTTTGGAATACAAAATTAGCATAAATTTAGTGAGGAAGATGGGACATCACTTCATTCTA
TTTTTTCGTTTCATTCAGTAATAAACAAAGAATATTCAAAGAACAAAACCGTATTCTCAC
GACTCCCATATAGACCGTTGTTTTAGTGGTGTAAAACACGATCAGAATATTTGGATATTA
TGTGTCAAAGATGTCCCATCTTCCCCCATCTACAGTATATATCCACGAATTCTTAGTAAA
GGCATTTAGCAGTTGGTCTTGCTTTTGGTGCCAAGTACTATATTATAAGTCTTACACTGT
TTACCATATACACTGTTTACGCTTTATACCAAATTTCCTATATATATTAGCCATGGTTGC
ACTAAACACACTATATAACAAAGCGTTCACTGACCAATCGCACGTATAATGAAGGAATGG
TTTACAAAGCATTGACTATTGCGAGGGATTTCCCAAATCGTTATCAGTTAAATAAATGCG
TAACTTTGAATGTTTTTATATGGACGACAATATGAACAACCCATGAGTTAGGTTAATGAC
CGTTAAGGCCGATTTTGCCCCAAAACACAAACATTTTGTCAGCGGCGTTTACAAAAGTTA
TACCCTTTATAGTTATGCAAATTAAGGTTACTTTCCCATAATATAAGTAATAAATTACGA
TGTAAAAATTTACAAAAAAAAATCGCGAAAGACGTTACTAAAGACTGCAACTAGTTTACT
ACAGTGATTATGTTTATAATGACTAATCGACAATAATTGTTAAATACCGAAACTTTGCAC
AACTGAATACAACTTACACATACGAGCCAATTTTTAGCTGAATTCTAATATCGCCTTATA
ATTTCTATACATATAATTAAAAATGCCGACTTAAACCGCTTATAGTATTTCAGCACCAAG
AGAATTTATTACAGCTGTTTAAAATGGGTTCAGTCATAGAACATTAGTTTTTGGTATATC
TATATTACGCAATACGTGACATAAGTGCTCGTGGTATGAAACTATTCACTACCCTTCGTA
ATGTTGACTATTTTCAATCCTAATACCACTCAATACAGTCATACTTACAATCACAGCACG
TTGTTTGTAGTGTAGACAATTAATAAACACTCAATATGATTTCAAAAAAACAAACGTTCT
GTAAATAGAATTTCAATTTAACCACTGTTGCTACTTGTATGTGTAACTATATAAAACTGC
ATCGCTTTTACGGTATGTAGTAGGGTGGGAGAAAGTGGGACACCTTTAGCGCATAATACC
CAAATAACCTGACCATGTTTCAAACAATTAACAACGGTCTATGGAAGTTGTGAGGATGTA
GTTTCATAATTCTTTTACTTTTCTTTTTTTACTACCAAATGGGACGACAAAGTTAACAGG
AGTCCCATCTTCCCCTACCCTACTGTATATATATTATGACTCTTCGTAATGCCAGCACTT
TACATTAAGTTTAATTTAAACTGCGAAACAGTTTATGGTTTAGGCTTTGAAAAGTGTGTG
CATGTGTAACTGTGACGAGTTGTCCCTTACGATGTATATTCTGACTGTTTGTATTACCGC
AGTAGTTAATAAAAAAATCAATTAAATACTAAACAGTTTGTTTTTCGCATAAATTAACAA
ATTCAGCAAAAATGCTTTGCGATATATACTCTGGCTTCAAATTACCAGCATGTTACAAGG
TCAAGGCAGTCACTGTAAGTGGCGATTATAGCACAGTCAACGTGCTAACATCGGTTCGTT
CGAGGTTGCAGCTTCATGCAGGCTAATAATGTGACAGCCGCTGCTTTGACAAGATCCAGG
GGCCAATACAAATCTTTATAAATAGGTAGCAGATATCTGTGCAAGCGACAGTCGAAGTGG
TCTTGGTTAAAGACATTGTTTAGCCTACCATCATAAGCCAGTGTGTCTGAGTGTCTTTTA
TAGACGTTTCACGAAATGTAACATATGTATAA

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60