Aniseed - Cis-regulation

Region 'REG00000502'

Cis-regulatory Region

Name

Ci-tropomyosin 3 -102/82 Tbx6-2 mutated

Short name

n/a

Region ID

REG00000502

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Christopher D. Brown (2009-12-09)

Annotator

Clara Degos (2009-12-09)

Curator

Delphine Dauga (2010-02-26)

View position in WashU

Description

Transcription start site is based on the start of the first exon of the KH transcript model. This region corresponds to construct 1504 in the paper. The second Tbx6 regulatory motif was mutated.

Hierarchy of Regulatory Regions

Modification of Ci-tropomyosin 3 -102/82 by substitution

Ci-tropomyosin 3 -2102/82 REG00000473 [ regulatory_region (extended_promoter) ]
- Ci-tropomyosin 3 -953/82 REG00000491 [ regulatory_region (extended_promoter) ]
- Ci-tropomyosin 3 -2102/-103 REG00000510 [ inactive_region ]

Regulated gene(s)

KH2012:KH.C3.661 (TPM1; TPM3; TPM4)

Construct(s) made to test this region

CONS00000473: pCi-tropomyosin 3 -102/82 Tbx6-2 mutated::LacZ

Show in situ data

Overview of functional TF binding sites in this region

4,367,4914,367,665

	Motif Name	Binding Factor(s)	Sequence	Position in Region
1	bZIP	n/a	TGACCTAA	[-82 / -75]
2	bZIP	n/a	TGACGTCA	[-70 / -63]
3	T-Box	n/a	TTATCACTT	[-58 / -50]

Sequence

Aniseed Coordinates: [4,367,491 / 4,367,665] on scaffold KhC3

Transcription start site is based on the start of the first exon of the KH transcript model. Tbx6-2 binding site mutated (TTCCTCACC) was replaced by "-".

Show sequence

Length: 184

---------TCTATATAGAATGACCTAATTGATGACGTCAAGCATTATCACTTCTACATT
GGCTATATAAGAGCGAAGTTGCCTTGTTATGCATCACACACAGTCGATTCTCACTCCACA
GCAGCACATTACTTCAACTAACTTGCAAAATGGAATCAATCAAGCACAAGATGGAAGGTC
TTAT

Reference(s)

Brown et al. (2007). Functional architecture and evolution of transcriptional elements that drive gene coexpression. Science 317(5844):1557-60